Somoza G M, Stéfano A, D'Eramo J L, Canosa L F, Fridman O
Departamento de Biotechnología Acuática, Fundación Centro de Investigaciones Médicas Albert Einstein, Buenos Aires, Argentina.
Gen Comp Endocrinol. 1994 Apr;94(1):44-52. doi: 10.1006/gcen.1994.1058.
Molecular variants of GnRH (gonadotropin-releasing hormone) in brain and pituitary extracts of the South American characiforme Prochilodus lineatus were studied using a combination of reverse-phase high-performance liquid chromatography and radioimmunoassay with different antisera. In brain extracts our study revealed that this fish has at least two different types of GnRH: cIIGnRH (chicken II) and sGnRH (salmon), and possibly a third variant of this molecule. In pituitary extracts we could find only two immunoreactive peaks corresponding to sGnRH and the possible third form.
利用反相高效液相色谱法和使用不同抗血清的放射免疫分析法相结合的方法，对南美脂鲤科鱼类细纹原唇齿脂鲤脑和垂体提取物中的促性腺激素释放激素（GnRH）分子变体进行了研究。在脑提取物中，我们的研究表明，这种鱼至少有两种不同类型的GnRH：cIIGnRH（鸡II型）和sGnRH（鲑鱼型），并且可能还有该分子的第三种变体。在垂体提取物中，我们仅发现了对应于sGnRH和可能的第三种形式的两个免疫反应峰。
