Kohut M L, Davis J M, Jackson D A, Jani P, Ghaffar A, Mayer E P, Essig D A
Department of Exercise Science, School of Public Health, and Department of Microbiology/Immunology, School of Medicine, University of South Carolina, Columbia, South Carolina 29208, USA.
Am J Physiol. 1998 Dec;275(6):L1089-94. doi: 10.1152/ajplung.1998.275.6.L1089.
Mice exercised to fatigue and exposed to herpes simplex virus type 1 (HSV-1) exhibit greater mortality than control mice. In this study, we examined lung macrophage resistance to HSV-1 after exercise in terms of both viral replication and interferon (IFN)-beta production. We utilized the reverse transcriptase-rapid polymerase chain reaction to measure the IFN-beta mRNA content in alveolar macrophages. IFN release was measured with a bioassay, and viral replication within the macrophage was assessed by plaque titration. Exercised (Ex) mice ran on a treadmill until fatigue while control (Con) mice remained in lanes above the treadmill. After exercise, alveolar macrophages were removed and incubated with HSV-1. Alveolar macrophage IFN-beta mRNA was greater in Ex than in Con mice. Culture supernatant from infected macrophages showed a higher degree of IFN release and a higher number of infectious viral particles in Ex vs. Con mice. It is likely that the increase in IFN-beta mRNA occurs in response to a higher degree of viral replication. These results suggest that macrophages from Ex mice are less resistant to infection with HSV-1.
运动至疲劳并暴露于1型单纯疱疹病毒（HSV-1）的小鼠比对照小鼠表现出更高的死亡率。在本研究中，我们从病毒复制和干扰素（IFN）-β产生两方面研究了运动后肺巨噬细胞对HSV-1的抵抗力。我们利用逆转录-快速聚合酶链反应来测量肺泡巨噬细胞中IFN-β mRNA的含量。通过生物测定法测量IFN释放，并通过噬斑滴定法评估巨噬细胞内的病毒复制。运动组（Ex）小鼠在跑步机上跑步直至疲劳，而对照组（Con）小鼠则留在跑步机上方的通道中。运动后，取出肺泡巨噬细胞并与HSV-1一起孵育。Ex组小鼠肺泡巨噬细胞的IFN-β mRNA高于Con组小鼠。与Con组小鼠相比，受感染巨噬细胞的培养上清液显示出更高程度的IFN释放和更多数量的感染性病毒颗粒。IFN-β mRNA的增加可能是对更高程度的病毒复制的反应。这些结果表明，Ex组小鼠的巨噬细胞对HSV-1感染的抵抗力较低。
