yTAFII61在RNA聚合酶II转录中发挥普遍作用，并且是Gcn4p招募SAGA共激活因子复合体所必需的。

yTAFII61 has a general role in RNA polymerase II transcription and is required by Gcn4p to recruit the SAGA coactivator complex.

作者信息

Natarajan K, Jackson B M, Rhee E, Hinnebusch A G

机构信息

Laboratory of Eukaryotic Gene Regulation, National Institute of Child Health and Human Development, Bethesda, Maryland 20892, USA.

出版信息

Mol Cell. 1998 Nov;2(5):683-92. doi: 10.1016/s1097-2765(00)80166-5.

DOI:10.1016/s1097-2765(00)80166-5

PMID:9844640

Abstract

We obtained a recessive insertion mutation in the gene encoding yeast TBP-associated factor yTAFII61/68 that impairs Gcn4p-independent and Gcn4p-activated HIS3 transcription. This mutation also reduces transcription of seven other class II genes, thus indicating a broad role for this yTAFII in RNA polymerase II transcription. The Gcn4p activation domain interacts with multiple components of the SAGA complex in cell extracts, including the yTAFII proteins associated with SAGA, but not with two yTAFIIs restricted to TFIID. The taf61-1 mutation impairs binding of Gcn4p to SAGA/yTAFII subunits but not to components of holoenzyme mediator. Our results provide strong evidence that recruitment of SAGA, in addition to holoenzyme, is crucial for activation by Gcn4p in vivo and that yTAFII61 plays a key role in this process.

摘要

我们在编码酵母TBP相关因子yTAFII61/68的基因中获得了一个隐性插入突变，该突变损害了不依赖Gcn4p和由Gcn4p激活的HIS3转录。此突变还降低了其他七个II类基因的转录，从而表明该yTAFII在RNA聚合酶II转录中具有广泛作用。Gcn4p激活域在细胞提取物中与SAGA复合物的多个组分相互作用，包括与SAGA相关的yTAFII蛋白，但不与局限于TFIID的两个yTAFII相互作用。taf61-1突变损害了Gcn4p与SAGA/yTAFII亚基的结合，但不影响其与全酶中介体组分的结合。我们的结果提供了强有力的证据，表明除全酶外，SAGA的募集对于Gcn4p在体内的激活至关重要，并且yTAFII61在此过程中起关键作用。