Park S, Kose T, Hamasaki M, Takeoka S, Nishide H, Tsuchida E
Department of Polymer Chemistry, Waseda University, Tokyo, Japan.
Artif Cells Blood Substit Immobil Biotechnol. 1998 Nov;26(5-6):497-506. doi: 10.3109/10731199809117470.
The hemoglobin vesicle (HbV) is a red cell substitute encapsulating purified concentrated Hb in a phospholipid vesicle. In order to improve the oxygen carrying capability of HbV, the pH value of the Hb solution should be adjusted to 7.0 in the HbV preparation, and then the pH value should be adjusted to 7.4 where HbV functions as an oxygen carrier, because the maximum value of [Hb]/[Lipid] was obtained in which the pH of the Hb solution was 7.0, and the metHb formation rate was suppressed in the pH 7.4. Generally, the pH control of the inner aqueous phase of HbV is difficult by changing the pH in the outer phase. We could control the pH of the Hb solution from 7.4 to 7.0 by dissolving CO2 into the Hb solution, and after the preparation of HbV, the pH of HbV is changed to 7.4 by reducing the pressure. The resulting pH-controlled HbV by CO2 gas showed a high [Hb]/[Lipid] value of 1.7 with a low rate of metHb formation.
血红蛋白囊泡（HbV）是一种红细胞替代物，它将纯化浓缩的血红蛋白包裹在磷脂囊泡中。为了提高HbV的携氧能力，在制备HbV时应将血红蛋白溶液的pH值调至7.0，然后在HbV作为氧载体发挥作用时将pH值调至7.4，因为当血红蛋白溶液的pH值为7.0时可获得最大的[Hb]/[脂质]值，且在pH 7.4时高铁血红蛋白的形成率受到抑制。一般来说，通过改变外相的pH值来控制HbV内水相的pH值是困难的。我们可以通过将二氧化碳溶解到血红蛋白溶液中将血红蛋白溶液的pH值从7.4调节到7.0，并且在制备HbV后，通过减压将HbV的pH值变为7.4。通过二氧化碳气体得到的pH受控的HbV显示出高的[Hb]/[脂质]值1.7，且高铁血红蛋白形成率低。
