Ohki N, Kimura T, Ogata Y
R&D Center, Terumo Corporation, Kanagawa, Japan.
Artif Cells Blood Substit Immobil Biotechnol. 1998 Nov;26(5-6):477-85. doi: 10.3109/10731199809117468.
The hemoglobin oxidation factors that produce methemoglobin (metHb) were studied, for example, loss of enzymatic activity, lack of electron carrier and effect of lipids. The adverse effect of lipids is very pronounced and must be eliminated as much as possible in the producing Neo Red Cell (NRC). The process of swelling was studied. Liquid for swelling was varied from water to basic liquids. The pH of swollen lipids varied from acidic to neutral or basic, metHb was decreased and the enzymes in the Embden-Meyerhof pathway were activated. Phosphatidylcholine (PC) which is a component of mixed lipids is decomposed to lisophosphatidylcholine (liso-PC) in basic pH liquids, but liso-PC formation was not detected when the pH of swollen lipids was under 7.8. For both metHb formation and PC decomposition, swelling with the same quantity of 0.2 N aqueous sodium hydroxide proved optimal.
对产生高铁血红蛋白（metHb）的血红蛋白氧化因子进行了研究，例如酶活性丧失、缺乏电子载体以及脂质的影响。脂质的不利影响非常明显，在生产新一代红细胞（NRC）时必须尽可能消除。研究了肿胀过程。用于肿胀的液体从水到碱性液体不等。肿胀脂质的pH值从酸性到中性或碱性变化，高铁血红蛋白减少，糖酵解途径中的酶被激活。作为混合脂质成分之一的磷脂酰胆碱（PC）在碱性pH液体中分解为溶血磷脂酰胆碱（liso-PC），但当肿胀脂质的pH值低于7.8时未检测到liso-PC的形成。对于高铁血红蛋白的形成和PC的分解，用相同量的0.2 N氢氧化钠水溶液进行肿胀证明是最佳的。
