单夹单肾高血压大鼠肾基底外侧膜中钠钾ATP酶活性的调节

Regulation of Na,K-ATPase activity in renal basolateral membrane of 1-clip-1-kidney hypertensive rat.

作者信息

Seok J H, Kim J B, Hong J H, Sung J Y, Hur G M, Lim K, Lee J H

机构信息

Department of Pharmacology and Biochemistry, College of Medicine, Chungnam National University, Taejon, Korea.

出版信息

Biochem Mol Biol Int. 1998 Nov;46(4):667-72. doi: 10.1080/15216549800204192.

DOI:10.1080/15216549800204192

PMID:9844726

Abstract

The changes of Na,K-ATPase activity and its regulation have been investigated in the renal cortex of 1-clip-1-kidney hypertensive rat. Ouabain-sensitive Na,K-ATPase activity (Emax) and [3H]ouabain-binding site (Bmax) in the hypertensive rat were slightly increased than those in the control. The levels of Na,K-ATPase alpha 1- and beta 1-subunit mRNA of the renal cortex in hypertensive rat were more increased than those in the control. Their increases were repressed by actinomycin-D, but not altered or more increased by cycloheximide. These results suggest that the increase of Na,K-ATPase activities and ouabain binding sites in 1-clip-1-kidney hypertensive rat may be correlated with the increases of gene expression in transcription level and/or of mRNA stability of Na,K-ATPase.

摘要

对单肾单夹高血压大鼠肾皮质中钠钾-ATP酶活性的变化及其调节进行了研究。高血压大鼠中哇巴因敏感的钠钾-ATP酶活性（Emax）和[3H]哇巴因结合位点（Bmax）比对照组略有增加。高血压大鼠肾皮质中钠钾-ATP酶α1和β1亚基mRNA的水平比对照组增加得更多。它们的增加被放线菌素-D抑制，但未被环己酰亚胺改变或进一步增加。这些结果表明，单肾单夹高血压大鼠中钠钾-ATP酶活性和哇巴因结合位点的增加可能与转录水平上基因表达的增加和/或钠钾-ATP酶mRNA稳定性的增加有关。