醛固酮刺激大鼠肾脏基底外侧膜中的钠钾ATP酶活性。

Aldosterone stimulates Na,K-ATPase activity in basolateral membrane of rat kidney.

作者信息

Seok J H, Kim J B, Hong J H, Hur G M, Jeon J R, Lim K, Hwang B D, Lee J H

机构信息

Department of Pharmacology and Biochemistry, College of Medicine, Chungnam National University, Taejon, Korea.

出版信息

Biochem Mol Biol Int. 1998 Aug;45(5):879-85. doi: 10.1002/iub.7510450505.

DOI:10.1002/iub.7510450505

PMID:9739452

Abstract

The changes of Na,K-ATPase activity and its regulation have been investigated in the renal cortex and its basolateral membrane of aldosterone-induced hypertensive rat. Ouabain-sensitive Na,K-ATPase activity and [3H]ouabain-binding site (Bmax) in the hypertensive rat were significantly increased than those in the control. The levels of Na,K-ATPase alpha 1- and beta 1-subunit mRNA of the renal cortex in hypertensive rat were more increased than those in the control, and their increases were repressed by actinomycin-D. These results suggest that the increase of Na,K-ATPase activities and ouabain binding sites in aldosterone-induced hypertensive rat may be correlated with transcriptional regulation of Na,K-ATPase gene expression.

摘要

在醛固酮诱导的高血压大鼠的肾皮质及其基底外侧膜中，对钠钾ATP酶活性的变化及其调节进行了研究。与对照组相比，高血压大鼠中哇巴因敏感的钠钾ATP酶活性和[3H]哇巴因结合位点（Bmax）显著增加。高血压大鼠肾皮质中钠钾ATP酶α1和β1亚基mRNA的水平比对照组增加得更多，并且它们的增加被放线菌素-D抑制。这些结果表明，醛固酮诱导的高血压大鼠中钠钾ATP酶活性和哇巴因结合位点的增加可能与钠钾ATP酶基因表达的转录调节相关。