黑腹果蝇中编码类泛素蛋白的SMT3 cDNA及基因的鉴定与表征
Identification and characterization of the SMT3 cDNA and gene encoding ubiquitin-like protein from Drosophila melanogaster.
作者信息
Huang H W, Tsoi S C, Sun Y H, Li S S
机构信息
Department of Biological Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan, ROC.
出版信息
Biochem Mol Biol Int. 1998 Nov;46(4):775-85. doi: 10.1080/15216549800204322.
A SMT3 cDNA encoding ubiquitin-like protein from Drosophila melanogaster was isolated and sequenced. Drosophila SMT3 genomic DNA was amplified by polymerase chain reaction, and its nucleotide sequence was found to be identical to that of the cDNA, indicating the absence of intron in its protein coding region. The sequence of 90 amino acids of Drosophila SMT3 exhibited 55%, 73%, 70% and 52% identity to yeast SMT3, human SMT3A, SMT3B and SMT3C protein.
从黑腹果蝇中分离并测序了一个编码泛素样蛋白的SMT3 cDNA。通过聚合酶链反应扩增了果蝇SMT3基因组DNA，发现其核苷酸序列与cDNA相同，表明其蛋白质编码区不存在内含子。果蝇SMT3的90个氨基酸序列与酵母SMT3、人SMT3A、SMT3B和SMT3C蛋白的同一性分别为55%、73%、70%和52%。
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