Qian Y C, Fan C Y, Gong Y, Yang S L
Shanghai Research Center of Biotechnology, CAS, P.R.China.
Biochem Mol Biol Int. 1998 Nov;46(4):821-8. doi: 10.1080/15216549800204362.
The cDNAs encoding six neurotoxin-like proteins were cloned from the total RNA isolated from one venom gland of Bungarus multicinctus multicinctus by reverse transcription-polymerase chain reaction. Although they shared a high degree of nucleotide sequence homology, the deduced proteins displayed a rather low extent of identical residues with the exception that the 21 residue signal peptides were highly conserved. Each mature protein consisted of 65, 66 or 68 amino acids with four or five disulfide bonds respectively. Comparative sequence analyses showed that they were structurally related to neurotoxin homologues. Phylogenetic analyses implied that they might represent two different evolutionary branches.
通过逆转录-聚合酶链反应，从多带带纹环蛇毒腺中分离的总RNA中克隆出编码六种神经毒素样蛋白的cDNA。尽管它们具有高度的核苷酸序列同源性，但推导的蛋白质除21个氨基酸的信号肽高度保守外，相同残基的程度相当低。每个成熟蛋白分别由65、66或68个氨基酸组成，分别具有四个或五个二硫键。比较序列分析表明，它们在结构上与神经毒素同系物相关。系统发育分析表明，它们可能代表两个不同的进化分支。
