Estepa J C, Aguilera-Tejero E, Mayer-Valor R, Almadén Y, Felsenfeld A J, Rodríguez M
Departamento de Medicina y Cirugia Animal, Universidad de Córdoba, Spain.
Equine Vet J. 1998 Nov;30(6):476-81. doi: 10.1111/j.2042-3306.1998.tb04522.x.
Measurement of parathyroid hormone (PTH) in horses was performed on plasma samples using 2 immunoradiometric assays: a human intact PTH assay and a rat amino-terminal PTH assay. The assays were validated by assessment of their precision, sensitivity and specificity, and also by evaluating PTH changes in the horse in response to variation in blood ionised calcium. Intra- and inter-assay variance, precision and sensitivity were similar for both human and rat assays; however, the rat assay was slightly more precise and sensitive than the human assay. Both assays detected an increase in PTH levels in the horse when blood ionised calcium was decreased and a decline in PTH concentration with hypercalcaemia. Measurement of PTH concentration in samples from healthy horses with the human assay yielded a mean (+/-s.e.) value of 31.3+/-4.1 pg/ml. When using the rat assay, PTH values were 44.1+/-5.3 pg/ml. Plasma samples held for up to 3 months at -20 degrees C did not show a significant change in PTH concentration. In conclusion, the human intact PTH and the rat amino-terminal assays detected equine PTH and can be used for measurement of this hormone in horses. Quantification of equine PTH using these assays will allow more precise diagnosis of a variety of disorders affecting mineral metabolism in horses.
使用两种免疫放射分析方法对马的血浆样本进行甲状旁腺激素（PTH）测定：一种是人类完整PTH测定法，另一种是大鼠氨基末端PTH测定法。通过评估其精密度、灵敏度和特异性，并通过评估马体内PTH随血液离子钙变化的情况，对这些测定方法进行了验证。人类和大鼠的测定方法在批内和批间差异、精密度和灵敏度方面相似；然而，大鼠测定法比人类测定法稍精确和灵敏。当血液离子钙降低时，两种测定方法均检测到马体内PTH水平升高，而高钙血症时PTH浓度下降。用人的测定方法对健康马的样本进行PTH浓度测定，得到的平均值（±标准误）为31.3±4.1 pg/ml。使用大鼠测定法时，PTH值为44.1±5.3 pg/ml。在-20℃下保存长达3个月的血浆样本，其PTH浓度未显示出显著变化。总之，人类完整PTH测定法和大鼠氨基末端测定法可检测马的PTH，可用于测定马体内的这种激素。使用这些测定方法对马的PTH进行定量将有助于更精确地诊断影响马矿物质代谢的各种疾病。
