Usenko V S, Lepekhin E A, Kornilovska I N, Lyzogubov V V, Apostolov E O, Ralets I S, Witt M
Morphological Laboratory BIONTEC, Dnepropetrovsk, Ukraine.
Anat Rec. 1998 Dec;252(4):600-7. doi: 10.1002/(SICI)1097-0185(199812)252:4<600::AID-AR10>3.0.CO;2-E.
The aim of this work was to study the effect of a dose of 150 microCi 131I on the barrier properties of the thyroid epithelium in pregnant female rats. Thirty-five female Wistar rats were divided into a control and four experimental groups (each distinguished by the time of 131I injection: group I--no less then 12 days before mating; groups II, III, and IV--on 5th, 10th, and 16th days of gestation, respectively). The thyroid glands were fixed in Bouin's fluid, embedded in paraffin, and stained immunohistochemically for thyroglobulin and fibronectin. In group IV the appearance of follicles with fibronectin-positive colloid demonstrates the penetration of blood plasma into the follicular lumen. There are more fibronectin positive follicles in group III. Regardless of the nature of the follicles' contents, numerous thyrocytes with an intensive fibronectin positive reaction begin to appear in the follicles. In group II the number of fibronectin positive follicles and thyrocytes is clearly reduced, and in group I only a few remain. In group IV there is a noticeable reduction in the quantity of colloid inside the follicles and often an absence of any thyroglobulin positive reaction. There are thyrocytes in which thyroglobulin positive granules localized in the basal zone. There is thyroglobulin positive staining in the stroma and blood vessels. In group II thyroglobulin is no longer found in the stroma. Small doses of 131I provoke a serious breakdown in the thyroid epithelium's barrier properties, although these changes are of a transient nature. The central zone of the thyroid gland reacts more actively and dynamically to exposure to radioactive iodine than the peripheral zone.
本研究旨在探讨150微居里¹³¹I剂量对妊娠雌性大鼠甲状腺上皮屏障特性的影响。将35只雌性Wistar大鼠分为对照组和四个实验组（每组根据¹³¹I注射时间区分：第一组——交配前不少于12天；第二、三、四组——分别在妊娠第5、10和16天）。甲状腺用Bouin氏液固定，石蜡包埋，免疫组织化学染色检测甲状腺球蛋白和纤连蛋白。在第四组中，出现纤连蛋白阳性胶体的滤泡表明血浆渗入滤泡腔。第三组中纤连蛋白阳性滤泡更多。无论滤泡内容物的性质如何，滤泡中开始出现大量纤连蛋白阳性反应强烈的甲状腺细胞。在第二组中，纤连蛋白阳性滤泡和甲状腺细胞数量明显减少，而在第一组中仅剩下少数。在第四组中，滤泡内胶体数量明显减少，且常常没有任何甲状腺球蛋白阳性反应。存在甲状腺球蛋白阳性颗粒位于基底区的甲状腺细胞。基质和血管中有甲状腺球蛋白阳性染色。在第二组中，基质中不再发现甲状腺球蛋白。小剂量¹³¹I会引发甲状腺上皮屏障特性的严重破坏，尽管这些变化是短暂的。甲状腺中央区对放射性碘暴露的反应比周边区更积极、更动态。
