单线态氧对细菌呼吸链酶的失活作用。

Inactivation of bacterial respiratory chain enzymes by singlet oxygen.

作者信息

Tatsuzawa H, Maruyama T, Misawa N, Fujimori K, Hori K, Sano Y, Kambayashi Y, Nakano M

机构信息

Shimizu Laboratories, Marine Biotechnology Institute, Japan.

出版信息

FEBS Lett. 1998 Nov 20;439(3):329-33. doi: 10.1016/s0014-5793(98)01397-0.

DOI:10.1016/s0014-5793(98)01397-0

PMID:9845348

Abstract

To distinguish the bactericidal action of singlet oxygen (1O2) from hypohalous acids, wild-type and lycopene transformant E. coli strains were exposed to each of the oxidants and then bacterial viability was investigated. 1O2 was generated by chemical and enzymatic systems at pH 4.5. ExpoSure of wild-type E. coli to 1O2 caused a significant loss of E. coli viability due to inactivation of membrane respiratory chain enzymes by 1O2. This action of 1O2 could be attenuated by lycopene in the bacterial cell membrane. In the lycopene transformant strain of E. coli, inactivation of NADH oxidase and succinate oxidase by hypohalous acids were significantly suppressed, but E. coli viability was unaffected. Based on these findings, we suggest that phagocytic leukocytes produce 1O2 as a major bactericidal oxidant in the phagosome.

摘要

为了区分单线态氧（¹O₂）与次卤酸的杀菌作用，将野生型和番茄红素转化型大肠杆菌菌株分别暴露于每种氧化剂中，然后研究细菌的活力。¹O₂在pH 4.5时通过化学和酶系统产生。野生型大肠杆菌暴露于¹O₂会导致大肠杆菌活力显著丧失，这是由于¹O₂使膜呼吸链酶失活所致。细菌细胞膜中的番茄红素可减弱¹O₂的这种作用。在大肠杆菌的番茄红素转化菌株中，次卤酸对NADH氧化酶和琥珀酸氧化酶的失活作用得到显著抑制，但大肠杆菌的活力未受影响。基于这些发现，我们认为吞噬性白细胞在吞噬小体中产生¹O₂作为主要的杀菌性氧化剂。