Pulido S M, Passaquin A C, Leijendekker W J, Challet C, Wallimann T, Rüegg U T
Pharmacology Group, School of Pharmacy, University of Lausanne, Switzerland.
FEBS Lett. 1998 Nov 20;439(3):357-62. doi: 10.1016/s0014-5793(98)01399-4.
Dystrophic skeletal muscle cells from Duchenne muscular dystrophy (DMD) patients and mdx mice exhibit elevated cytosolic Ca2+ concentrations ([Ca2+]c). Pretreatment of mdr myotubes for 6-12 days with creatine (20 mM) decreased the elevation in [Ca2+]c induced by either high extracellular Ca2+ concentrations or hypo-osmotic stress to control levels. 45Ca2+ influx measurements suggest that creatine lowered [Ca2+]c by stimulating sarcoplasmic reticulum Ca2+-ATPase. Creatine pretreatment increased levels of phosphocreatine but not ATP. Furthermore, myotube formation and survival were significantly enhanced by creatine pretreatment. Therefore, creatine supplementation may be useful for treatment of DMD.
来自杜兴氏肌肉营养不良症（DMD）患者和mdx小鼠的营养不良性骨骼肌细胞表现出细胞溶质Ca2+浓度（[Ca2+]c）升高。用肌酸（20 mM）对mdr肌管进行6 - 12天的预处理，可将高细胞外Ca2+浓度或低渗应激诱导的[Ca2+]c升高降低至对照水平。45Ca2+流入测量表明，肌酸通过刺激肌浆网Ca2+-ATPase降低了[Ca2+]c。肌酸预处理增加了磷酸肌酸水平，但未增加ATP水平。此外，肌酸预处理显著增强了肌管形成和存活。因此，补充肌酸可能对治疗DMD有用。
