Holstege F C, Jennings E G, Wyrick J J, Lee T I, Hengartner C J, Green M R, Golub T R, Lander E S, Young R A
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA.
Cell. 1998 Nov 25;95(5):717-28. doi: 10.1016/s0092-8674(00)81641-4.
Genome-wide expression analysis was used to identify genes whose expression depends on the functions of key components of the transcription initiation machinery in yeast. Components of the RNA polymerase II holoenzyme, the general transcription factor TFIID, and the SAGA chromatin modification complex were found to have roles in expression of distinct sets of genes. The results reveal an unanticipated level of regulation which is superimposed on that due to gene-specific transcription factors, a novel mechanism for coordinate regulation of specific sets of genes when cells encounter limiting nutrients, and evidence that the ultimate targets of signal transduction pathways can be identified within the initiation apparatus.
全基因组表达分析用于鉴定那些其表达依赖于酵母中转录起始机制关键组分功能的基因。发现RNA聚合酶II全酶、通用转录因子TFIID以及SAGA染色质修饰复合体的组分在不同基因集的表达中发挥作用。这些结果揭示了一种意想不到的调控水平，它叠加于由基因特异性转录因子引起的调控之上，是一种细胞遇到营养限制时特定基因集协同调控的新机制，以及信号转导途径的最终靶点可在起始装置中被识别的证据。
