Zuberi A R, Christianson G J, Mendoza L M, Shastri N, Roopenian D C
The Jackson Laboratory, Bar Harbor, Maine 04609, USA.
Immunity. 1998 Nov;9(5):687-98. doi: 10.1016/s1074-7613(00)80666-4.
Immune responses to minor histocompatibility antigens are poorly understood and present substantial barriers to successful solid tissue and bone marrow transplantation among MHC-matched individuals. We exploited a unique positional cloning approach relying on the potent negative selection capability of cytotoxic T cells to identify the H3a gene responsible for immunodominant H2-Db-restricted determinants of the classically defined mouse autosomal H3 complex. The allelic basis for reciprocal H3a antigens is two amino acid changes within a single nonamer H2-Db-binding peptide. The H3a gene, now called Zfp106, encodes a 1888-amino acid protein with three zinc fingers and a beta-transducin domain consistent with DNA/protein binding. A region of ZFP106 is identical to a 600-amino acid sequence implicated in the insulin receptor signaling pathway.
对次要组织相容性抗原的免疫反应了解甚少，并且在MHC匹配个体之间成功进行实体组织和骨髓移植时构成了重大障碍。我们利用了一种独特的定位克隆方法，该方法依赖于细胞毒性T细胞强大的阴性选择能力，以鉴定负责经典定义的小鼠常染色体H3复合体免疫显性H2-Db限制性决定簇的H3a基因。相互H3a抗原的等位基因基础是单个九聚体H2-Db结合肽内的两个氨基酸变化。H3a基因，现称为Zfp106，编码一种含有三个锌指和一个与DNA/蛋白质结合一致的β-转导蛋白结构域的1888个氨基酸的蛋白质。ZFP106的一个区域与胰岛素受体信号通路中涉及的一个600个氨基酸的序列相同。
