Puglisi E V, Puglisi J D
Department of Structural Biology, Stanford University School of Medicine, California 94305-5400, USA.
Nat Struct Biol. 1998 Dec;5(12):1033-6. doi: 10.1038/4141.
Interaction of HIV-1 genomic RNA and human tRNA(Lys)3 initiates viral reverse transcription. An adenosine-rich (A-rich) loop in HIV RNA mediates complex formation between tRNA and viral RNA. We have determined the structure of an A-rich loop oligonucleotide using nuclear magnetic resonance spectroscopy. The loop structure is stabilized by a noncanonical G-A pair and a U-turn motif, which leads to stacking of the conserved adenosines. The structure has similarity to the tRNA anticodon structure, and suggests possible mechanisms for its role in initiation of reverse transcription.
HIV-1基因组RNA与人类tRNA(Lys)3的相互作用启动病毒逆转录。HIV RNA中富含腺苷（A-rich）的环介导了tRNA与病毒RNA之间的复合物形成。我们利用核磁共振光谱法确定了富含A的环寡核苷酸的结构。该环结构由一个非经典的G-A对和一个U型转弯基序稳定，这导致了保守腺苷的堆积。该结构与tRNA反密码子结构相似，并提示了其在逆转录起始中发挥作用的可能机制。
