Virchow J C, Julius P, Lommatzsch M, Luttmann W, Renz H, Braun A
Institute of Laboratory Medicine and Pathobiochemistry, Charité-Campus Virchow-Klinikum, Humboldt University, Berlin, Germany.
Am J Respir Crit Care Med. 1998 Dec;158(6):2002-5. doi: 10.1164/ajrccm.158.6.9803023.
The mechanisms linking inflammation and airway hyperresponsiveness in allergic bronchial asthma are still not completely defined. Since neurotrophic factors increase nerve excitability and neurotransmitter synthesis and are produced by immunocompetent cells, they are likely candidates as mediators of inflammation and hyperresponsiveness. We tested the hypothesis that neurotrophin concentrations will increase in the bronchoalveolar lavage (BAL) fluid from patients with asthma after segmental allergen provocation. For this purpose an individually standardized dose of allergen or saline was instilled into different segments during bronchoscopy in eight subjects with mild allergic bronchial asthma. Segments were then lavaged 10 min and 18 h after allergen challenge or saline instillation. There was a significant increase in the neurotrophins nerve growth factor, brain-derived neurotrophic factor, and neurotrophin-3 in BAL fluids 18 h after allergen but not saline challenge. We conclude that neurotrophins are produced endobronchially following allergen provocation, suggesting a contribution to the pathogenesis of asthma.
变应性支气管哮喘中炎症与气道高反应性之间的联系机制仍未完全明确。由于神经营养因子可提高神经兴奋性并促进神经递质合成，且由免疫活性细胞产生，因此它们很可能是炎症和高反应性的介质。我们检验了这样一个假设：在节段性变应原激发后，哮喘患者支气管肺泡灌洗（BAL）液中的神经营养因子浓度会升高。为此，在支气管镜检查期间，向8名轻度变应性支气管哮喘患者的不同节段注入个体化标准化剂量的变应原或生理盐水。在变应原激发或生理盐水注入后10分钟和18小时，对各节段进行灌洗。变应原激发后18小时，BAL液中的神经营养因子神经生长因子、脑源性神经营养因子和神经营养素-3显著增加，而生理盐水激发后则无此现象。我们得出结论，变应原激发后支气管内会产生神经营养因子，提示其对哮喘发病机制有一定作用。
