Abergel C, Robertson D L, Claverie J M
Laboratory of Structural and Genetic Information, CNRS EP-91, Marseille F-13402, France.
J Virol. 1999 Jan;73(1):751-3. doi: 10.1128/JVI.73.1.751-753.1999.
A coding region homologous to the sequence for essential eukaryotic enzyme dUTPase has been identified in different genomic regions of several viral lineages. Unlike the nonprimate lentiviruses (caprine arthritis- encephalitis virus, equine infectious anemia virus, feline immunodeficiency virus, and visna virus), where dUTPase is integrated into the pol coding region, this enzyme has never been demonstrated to be present in the primate lentivirus genomes (human immunodeficiency virus type 1 [HIV-1], HIV-2, or the related simian immunodeficiency virus). A novel approach allowed us to identify a weak but significant sequence similarity between HIV-1 gp120 and the human dUTPase. This finding was then extended to all of the primate lentivirus lineages. Together with the recently reported fragmentary structural similarity between the V3 loop region and the Escherichia coli dUTPase (P. D. Kwong, R. Wyatt, J. Robinson, R. W. Sweet, J. Sodroski, and W. A. Hendrickson, Nature 393:648-659, 1998), our results strongly suggest that an ancestral dUTPase gene has evolved into the present primate lentivirus CD4 and cytokine receptor interacting region of gp120.
在几种病毒谱系的不同基因组区域中，已鉴定出一个与真核生物必需酶dUTPase序列同源的编码区。与非灵长类慢病毒（山羊关节炎-脑炎病毒、马传染性贫血病毒、猫免疫缺陷病毒和维斯纳病毒）不同，在非灵长类慢病毒中dUTPase被整合到pol编码区，而在灵长类慢病毒基因组（1型人类免疫缺陷病毒[HIV-1]、HIV-2或相关的猴免疫缺陷病毒）中从未证实存在这种酶。一种新方法使我们能够鉴定出HIV-1 gp120与人dUTPase之间微弱但显著的序列相似性。这一发现随后扩展到所有灵长类慢病毒谱系。连同最近报道的V3环区域与大肠杆菌dUTPase之间的片段性结构相似性（P.D.邝、R.怀亚特、J.罗宾逊、R.W.斯威特、J.索德罗斯基和W.A.亨德里克森，《自然》393:648 - 659，1998），我们的结果强烈表明，一个祖先dUTPase基因已进化为目前灵长类慢病毒gp120的CD4和细胞因子受体相互作用区域。
