猫杯状病毒衣壳蛋白在猫培养细胞中的表达及衣壳组装
Feline calicivirus capsid protein expression and capsid assembly in cultured feline cells.
作者信息
Geissler K, Schneider K, Fleuchaus A, Parrish C R, Sutter G, Truyen U
机构信息
Institute for Medical Microbiology, Infectious and Epidemic Diseases, Ludwig Maximilians University Munich, 80539 Munich, Germany.
出版信息
J Virol. 1999 Jan;73(1):834-8. doi: 10.1128/JVI.73.1.834-838.1999.
Abstract
The capsid protein of feline calicivirus (FCV) was expressed by using plasmids containing cytomegalovirus, simian virus 40, or T7 promoters. The strongest expression was achieved with the T7 promoter and coinfection with vaccinia virus expressing the T7 RNA polymerase (MVA/T7pol). The FCV precursor capsid protein was processed to the mature-size protein, and these proteins were assembled in to virus-like particles.
摘要
通过使用含有巨细胞病毒、猿猴病毒40或T7启动子的质粒来表达猫杯状病毒（FCV）的衣壳蛋白。使用T7启动子并与表达T7 RNA聚合酶的痘苗病毒（MVA/T7pol）共感染时，可实现最强表达。FCV前体衣壳蛋白被加工成成熟大小的蛋白，并且这些蛋白组装成病毒样颗粒。
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