Pai K, Yanagawa H, Takauchi E, Suzuki Y, Hanibuchi M, Parajuli P, Sone S
Third Department of Internal Medicine, University of Tokushima School of Medicine, Japan.
Oncol Res. 1998;10(6):295-300.
We investigated the regulatory effect of interleukin (IL) 10 on IL-12-inducible killer activity of blood mononuclear cells (MNC) of healthy subjects. IL-10 suppressed non-MHC-restricted killer activity induced by IL-12 in a dose-dependent manner. Cytotoxicity against Daudi cells of blood MNC activated with optimal dose (100 U/ml) of IL-12 was significantly suppressed from 36.6 +/- 6.8% (mean +/- SE) to 26.0 +/- 5.0% by 100 ng/ml of IL-10 (n = 5; P < 0.05) at an E/T ratio of 20. IL-10, however, did not suppress killer induction by IL-2 from blood MNC. On the other hand, IL-4, a known inhibitor of IL-2-mediated killer induction, had no effect on IL-12-mediated killer induction from blood MNC. Moreover, production of interferon-gamma by IL-12-stimulated MNC was suppressed by IL-10. Because the presence of IL-10 is reported in the tumor-growing site, these observations suggest that IL-10 acts as a key cytokine in the suppression of IL-12-mediated antitumor response in situ.
我们研究了白细胞介素（IL）-10对健康受试者血液单核细胞（MNC）的IL-12诱导杀伤活性的调节作用。IL-10以剂量依赖的方式抑制IL-12诱导的非主要组织相容性复合体（MHC）限制的杀伤活性。在E/T比值为20时，100 ng/ml的IL-10可将用最佳剂量（100 U/ml）的IL-12激活的血液MNC对Daudi细胞的细胞毒性从36.6±6.8%（平均值±标准误）显著抑制至26.0±5.0%（n = 5；P < 0.05）。然而，IL-10并未抑制血液MNC中IL-2诱导的杀伤作用。另一方面，已知的IL-2介导的杀伤诱导抑制剂IL-4对血液MNC中IL-12介导的杀伤诱导没有影响。此外，IL-10抑制了IL-12刺激的MNC产生干扰素-γ。由于在肿瘤生长部位报道了IL-10的存在，这些观察结果表明IL-10在原位抑制IL-12介导的抗肿瘤反应中起关键细胞因子的作用。
