Son Y I, Dallal R M, Mailliard R B, Egawa S, Jonak Z L, Lotze M T
Department of Surgery, University of Pittsburgh Cancer Institute, Pennsylvania 15261, USA.
Cancer Res. 2001 Feb 1;61(3):884-8.
We investigated the in vitro effects of combining interleukin-18 (IL-18) and IL-2 on human lymphocytes. The combined use of these two cytokines synergistically enhanced the proliferation, cytolytic activity, and interferon-gamma production of peripheral blood mononuclear cells. Phenotypic analysis revealed a preferential expansion of CD56+CD3- cells and an up-regulation of IL-2 receptor-alpha expression on natural killer cells. Isolated natural killer cells showed a substantial increase in proliferation and cytotoxicity compared with CD4+ and CD8+ T cells. The combined use of IL-18 and IL-2 should be considered a viable strategy to induce an antitumor response in vivo.
我们研究了白细胞介素-18（IL-18）与IL-2联合应用对人淋巴细胞的体外作用。这两种细胞因子联合使用可协同增强外周血单个核细胞的增殖、细胞溶解活性及γ干扰素的产生。表型分析显示CD56+CD3-细胞优先扩增，且自然杀伤细胞上IL-2受体α表达上调。与CD4+和CD8+ T细胞相比，分离出的自然杀伤细胞在增殖和细胞毒性方面有显著增加。IL-18与IL-2联合使用应被视为在体内诱导抗肿瘤反应的可行策略。
