Matyshevs'ka O P
Taras Shevchenko Kyiv University.
Ukr Biokhim Zh (1978). 1998 Jul-Aug;70(4):94-100.
Stimulation of 32P-phosphoinositides turnover and phosphatidylinositol-4,5-biphosphate hydrolysis is observed 6 h after animals X-ray irradiation in a dose 1 Gy. Both mitogenic lectins LPS and PHA (10 mg/ml), but not 0.5 microM A23187 induce inositol phosphates release from endogenous [3H]inositol--phospholipids in lymphocytes. The level of inositol triphosphate is increased within 1-6 h and decreased in 12 h after irradiation. 45Ca2+ influx through lymphocytes plasma membrane is not changed within 3 h, but increases greatly 12 h after exposure. Data obtained indicate that IP3 formation is an early signal for radiation-induced changes in calcium homeostasis.
在动物接受1 Gy剂量的X射线照射6小时后，可观察到32P - 磷酸肌醇周转率和磷脂酰肌醇 - 4,5 - 二磷酸水解。促有丝分裂凝集素LPS和PHA（10 mg/ml），但0.5 microM A23187不会诱导淋巴细胞内源性[3H]肌醇 - 磷脂释放肌醇磷酸。照射后1 - 6小时内肌醇三磷酸水平升高，12小时后降低。照射后3小时内，通过淋巴细胞质膜的45Ca2+内流没有变化，但照射12小时后大幅增加。所获得的数据表明，IP3的形成是辐射诱导钙稳态变化的早期信号。
