Luna V A, Roberts M C
Department of Pathobiology, University of Washington, Seattle 98195, USA.
J Antimicrob Chemother. 1998 Nov;42(5):613-9. doi: 10.1093/jac/42.5.613.
Seventy tetracycline-resistant Streptococcus pneumoniae were tested for the presence of tetracycline resistance genes, tetM and tetO, using a polymerase chain reaction (PCR) assay and DNA-DNA hybridization. Seven isolates representing five serotypes (12, 22, 6A, 19F and 23) carried the tetO gene. Five of the isolates were genetically unrelated as judged using pulsed field gel electrophoresis (PFGE) analysis. Two 19F isolates came from the same patient, carried both tetM and tetO genes and had the same PFGE pattern. The other 63 isolates carried only the tetM gene. DNA sequences from three of the tetO-carrying isolates were determined; they showed 91-95% nucleotide sequence identity over 300 nucleotides, and 93-95% amino acid sequence identity over 100 amino acids. The isolates carrying both tetO and tetM genes could transfer the tetM gene into both Enterococcus faecalis and S. pneumoniae recipients, but not the tetO gene. There was no detectable transfer of the tetO gene, by conjugation, from the other five isolates.
使用聚合酶链反应（PCR）检测法和DNA-DNA杂交技术，对70株四环素耐药肺炎链球菌进行四环素耐药基因tetM和tetO检测。代表5种血清型（12、22、6A、19F和23）的7株分离株携带tetO基因。根据脉冲场凝胶电泳（PFGE）分析判断，其中5株分离株在基因上不相关。2株19F分离株来自同一患者，携带tetM和tetO基因，且具有相同的PFGE图谱。其他63株分离株仅携带tetM基因。测定了3株携带tetO分离株的DNA序列；它们在300个核苷酸上显示出91%-95%的核苷酸序列同一性，在100个氨基酸上显示出93%-95%的氨基酸序列同一性。同时携带tetO和tetM基因的分离株可将tetM基因转移至粪肠球菌和肺炎链球菌受体中，但不能转移tetO基因。通过接合作用，未检测到其他5株分离株的tetO基因发生转移。
Zotero 插件