Isenberg D A, McClure C, Farewell V, Spellerberg M, Williams W, Cambridge G, Stevenson F
Centre For Rheumatology/Bloomsbury Rheumatology Unit, University College London.
Ann Rheum Dis. 1998 Sep;57(9):566-70. doi: 10.1136/ard.57.9.566.
To compare the levels of the 9G4 idiotope (9G4 Id) in systemic lupus erythematosus (SLE) patients with a detailed disease activity index, the British Isles Lupus Assessment Group (BILAG) index, and serological parameters of disease activity by ds DNA antibody levels and serum C3 concentrations.
In a cross sectional analysis serum samples from 190 patients with SLE were studied and a further 55 serial bleeds from 14 patients. An enzyme linked immunosorbent assay was used to measure the 9G4 Id, and anti dsDNA and antimyeloperoxidase (MPO) antibodies. The C3 levels were measured by laser nephelometer.
Seventy six of 190 (40%) of the patients tested had raised 9G4 Id levels. In the cross sectional study 9G4 Id levels were found to correlate with disease activity in the BILAG cardiovascular/respiratory renal, and haematological systems and with global BILAG score (p < 0.01). In the serial bleeds 9G4 Id levels correlated with anti-dsDNA antibody and C3 levels, but not with anti-MPO antibodies. No correlations were found with treatment. In six cases the 9G4 Id levels correlated well with global BILAG scores and dsDNA antibody levels. In four cases the BILAG global and 9G4 Id levels alone correlated well.
Raised levels of the 9G4 Id are present in a substantial proportion of serum samples from patients with lupus, correlate with various aspects of disease activity in SLE. The Id is detectable on anti-dsDNA antibodies, though it must also be present on other immunoglobulins whose specificities remain unknown.
通过详细的疾病活动指数——英伦三岛狼疮评估组（BILAG）指数以及疾病活动的血清学参数（双链DNA抗体水平和血清C3浓度），比较系统性红斑狼疮（SLE）患者体内9G4独特型（9G4 Id）的水平。
在一项横断面分析中，研究了190例SLE患者的血清样本，并对另外14例患者的55份连续血样进行了研究。采用酶联免疫吸附测定法检测9G4 Id、抗双链DNA抗体和抗髓过氧化物酶（MPO）抗体。通过激光散射比浊法测定C3水平。
190例接受检测的患者中有76例（40%）的9G4 Id水平升高。在横断面研究中，发现9G4 Id水平与BILAG心血管/呼吸、肾脏及血液系统的疾病活动以及BILAG总体评分相关（p<0.01）。在连续血样检测中，9G4 Id水平与抗双链DNA抗体和C3水平相关，但与抗MPO抗体无关。未发现与治疗存在相关性。在6例患者中，9G4 Id水平与BILAG总体评分及双链DNA抗体水平相关性良好。在4例患者中，仅BILAG总体评分与9G4 Id水平相关性良好。
狼疮患者的血清样本中，相当一部分存在9G4 Id水平升高的情况，其与SLE疾病活动的各个方面相关。该独特型可在抗双链DNA抗体上检测到，不过它也必定存在于其他特异性尚不清楚的免疫球蛋白上。
