细胞色素P450 3A4在人胃小凹上皮肠化生中的表达。
Expression of cytochrome P450 3A4 in foveolar epithelium with intestinal metaplasia of the human stomach.
作者信息
Yokose T, Doy M, Kakiki M, Horie T, Matsuzaki Y, Mukai K
机构信息
Pathology Division, National Cancer Center Research Institute East, Chiba.
出版信息
Jpn J Cancer Res. 1998 Oct;89(10):1028-32. doi: 10.1111/j.1349-7006.1998.tb00492.x.
The expression of cytochrome P450 3A4 (CYP3A4) in the foveolar epithelium of the human stomach with intestinal metaplasia was studied using immunohistochemistry, western blotting and reverse transcription polymerase chain reaction (RT-PCR). CYP3A4 was immunohistochemically detected in the foveolar epithelium with intestinal metaplasia, but was not detected in foveolar epithelium without intestinal metaplasia, in the pyloric gland or in the fundic gland of the stomach. Western blotting and RT-PCR demonstrated that CYP3A4 protein and mRNA were expressed in the liver and pyloric gland mucosa with intestinal metaplasia, but not in the fundic gland mucosa without intestinal metaplasia. Possible roles of CYP expression in the gastric mucosa with intestinal metaplasia in human stomach carcinogenesis are briefly discussed.
利用免疫组织化学、蛋白质印迹法和逆转录聚合酶链反应（RT-PCR）研究了细胞色素P450 3A4（CYP3A4）在人胃肠化生的小凹上皮中的表达。免疫组织化学检测发现，CYP3A4在有肠化生的小凹上皮中存在，但在无肠化生的小凹上皮、胃幽门腺或胃底腺中未检测到。蛋白质印迹法和RT-PCR表明，CYP3A4蛋白和mRNA在有肠化生的肝脏和幽门腺黏膜中表达，但在无肠化生的胃底腺黏膜中不表达。本文简要讨论了CYP表达在人胃肠化生胃黏膜致癌过程中的可能作用。
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