Uguru M O, Okwuasaba F K, Ekwenchi E E, Uguru V E
Department of Pharmacology and Clinical Pharmacy, University of Jos, Nigeria.
J Ethnopharmacol. 1998 Oct;62(3):203-8. doi: 10.1016/s0378-8741(98)00037-3.
The oxytocic activity of the hot methanol extract (HME) of the leaves of Monechma ciliatum was compared with other uterine stimulants like ergometrine, oxytocin, 5-hydroxytryptamine (5-HT), acetylcholine (ACh) and prostaglandins (PGs) E2 and F2alpha (PGE2 and PGF2alpha) in the presence of some antagonists in an attempt to explain the mechanism of action of the extract. The effects of the reference drugs on uteri isolated from rats pretreated with HME for 2 weeks were also observed. Atropine blocked the effect of ACh and partially blocked those of HME while L-366-948 blocked only the effect of oxytocin. Indomethacin inhibited the effects of HME as well as all the other drugs, except the PGs and ACh. D-600 blocked the effect of all the drugs including HME. Methysergide antagonised only the effect of 5-HT and partially blocked ergometrine. Prolonged treatment altered the uterine musculature and the activity profile of the reference drugs. These results suggest that the HME may be acting by more than one mechanism to contract the uterus and explains the mechanism of the anti-implantation activity of the plant.
将纤毛独脚金属植物叶片的热甲醇提取物（HME）的催产活性与其他子宫兴奋剂如麦角新碱、催产素、5-羟色胺（5-HT）、乙酰胆碱（ACh）以及前列腺素（PGs）E2和F2α（PGE2和PGF2α）在一些拮抗剂存在的情况下进行比较，以试图解释该提取物的作用机制。还观察了参考药物对用HME预处理2周的大鼠分离子宫的影响。阿托品阻断了ACh的作用并部分阻断了HME的作用，而L-366-948仅阻断了催产素的作用。吲哚美辛抑制了HME以及所有其他药物的作用，但PGs和ACh除外。D-600阻断了包括HME在内的所有药物的作用。麦角酰二乙胺仅拮抗5-HT的作用并部分阻断麦角新碱。长期治疗改变了子宫肌肉组织和参考药物的活性特征。这些结果表明，HME可能通过多种机制作用于子宫收缩，并解释了该植物抗着床活性的机制。
