内皮素-1对正常孕妇和先兆子痫孕妇子宫肌条自发收缩的影响以及尼莫地平和伊拉地平对内皮素-1诱导收缩的影响。

Effect of endothelin-1 on spontaneous contractions and effects of nimodipine and isradipine on endothelin-1-induced contractions in myometrial strips isolated from normal pregnant and preeclamptic women.

作者信息

Kaya T, Cetin A, Cetin M, Sarioglu Y

机构信息

Department of Pharmacology, Cumhuriyet University School of Medicine, Sivas, Turkey.

出版信息

Acta Obstet Gynecol Scand. 1998 Nov;77(10):961-6.

PMID:9849837

Abstract

BACKGROUND

To examine the effect of endothelin-1 (ET-1) on spontaneous contractile activity and the effects of nimodipine and isradipine on ET-1-induced contractile responses in myometrial strips isolated from normal pregnant and preeclamptic women.

MATERIAL AND METHODS

. Isolated myometrial strips were obtained from seven normal pregnant and seven preeclamptic women undergoing elective cesarean section and the strips were mounted in organ baths for recording of isometric tension. The effect of increasing concentration of ET-1 on spontaneous contractions and effects of increasing concentration of nimodipine and isradipine on ET-1-induced contractions were recorded.

RESULTS

ET-1 dose-dependently (10(-11)-10(-8) M) increased the amplitude and frequency of spontaneous contractions in all myometrial strips obtained from normal pregnant and preeclamptic women. The increase in the amplitude of contractions in preeclamptic strips was significantly higher than that of normal strips. The increase in amplitude of contractions in normal and preeclamptic strips reached statistical significance beginning from the concentrations of 10(-9) M and 10(-11) M, respectively. ET-1 (10(-8) M) also increased the basal tone of all myometrial strips isolated from normal pregnant and preeclamptic women. When ET-1 (10(-8) M)-contracted myoinetrial strips were exposed to increasing concentrations of nimodipine (10(-6)-3x10(-5)M) and isradipine (10(-5)-3x10(-4) M), nimodipine and isradipine dose-dependently decreased the amplitude and frequency of contractions.

CONCLUSIONS

The increase in contractile response with ET-1 was significantly higher in myometrial strips isolated from preeclamptic women compared to those of myometrial strips isolated from normal pregnant women. These increases in contractile response are at least in part mediated by dihydropyridine-sensitive calcium channels, since they were significantly reduced in the presence of increasing concentrations of nimodipine and isradipine.

摘要

背景

研究内皮素-1（ET-1）对正常孕妇和子痫前期孕妇离体子宫肌条自发收缩活动的影响，以及尼莫地平和伊拉地平对ET-1诱导的收缩反应的影响。

材料与方法

从7例择期剖宫产的正常孕妇和7例子痫前期孕妇获取离体子宫肌条，将肌条置于器官浴槽中记录等长张力。记录ET-1浓度增加对自发收缩的影响以及尼莫地平和伊拉地平浓度增加对ET-1诱导收缩的影响。

结果

ET-1（10⁻¹¹ - 10⁻⁸ M）剂量依赖性地增加了从正常孕妇和子痫前期孕妇获取的所有子宫肌条的自发收缩幅度和频率。子痫前期肌条收缩幅度的增加显著高于正常肌条。正常和子痫前期肌条收缩幅度的增加分别从10⁻⁹ M和10⁻¹¹ M浓度开始具有统计学意义。ET-1（10⁻⁸ M）也增加了从正常孕妇和子痫前期孕妇分离的所有子宫肌条的基础张力。当ET-1（10⁻⁸ M）收缩的子宫肌条暴露于浓度增加的尼莫地平（10⁻⁶ - 3×10⁻⁵ M）和伊拉地平（10⁻⁵ - 3×10⁻⁴ M）时，尼莫地平和伊拉地平剂量依赖性地降低了收缩幅度和频率。