Morris E, Kirwan J F, Sujatha S, Rostron C K
St George's Hospital, London, UK.
Eye (Lond). 1998;12 ( Pt 4):619-22. doi: 10.1038/eye.1998.155.
To assess the corneal endothelial cell density following deep lamellar keratoplasty (DLK) carried out by air dissection and with the transplantation of lyophilised tissue.
Contact endothelial specular microscopy was carried out on a series of patients who had undergone DLK with a minimum of 1 year post-operative follow-up.
Twenty eyes of 18 patients were examined at follow-up times ranging from 1 to 8 years post-operatively (mean 3 years). Indications for surgery were: keratoconus (n = 12), herpes simplex virus (HSV) keratitis (n = 1), herpes zoster ophthalmicus (HZO) keratitis (n = 1), lipid keratopathy (n = 2), lattice dystrophy (n = 1) and corneal scarring (n = 3). Overall, mean post-operative cell density was 2417 cells/mm2 (range 928-3656 cells/mm2). In eyes with pathological conditions not likely to have affected the endothelial cell density, such as keratoconus or lattice degeneration, the mean cell density was 2837 cells/mm2 (range 1030-3656 cells/mm2).
In patients undergoing DLK for conditions such as keratoconus the post-operative cell density was at a normal level in the majority of cases. Cell loss through this surgical intervention thus appears generally to be small. The prospect for long-term survival of these grafts is good.
评估采用空气分离法并移植冻干组织进行深板层角膜移植术（DLK）后的角膜内皮细胞密度。
对一系列接受DLK且术后随访至少1年的患者进行接触式内皮细胞镜检查。
在术后1至8年（平均3年）的随访期内，对18例患者的20只眼睛进行了检查。手术适应症包括：圆锥角膜（n = 12）、单纯疱疹病毒（HSV）角膜炎（n = 1）、带状疱疹性角膜炎（HZO）（n = 1）、脂质角膜病变（n = 2）、格子状营养不良（n = 1）和角膜瘢痕（n = 3）。总体而言，术后平均细胞密度为2417个细胞/mm²（范围为928 - 3656个细胞/mm²）。在不太可能影响内皮细胞密度的病理状况的眼睛中，如圆锥角膜或格子状变性，平均细胞密度为2837个细胞/mm²（范围为1030 - 3656个细胞/mm²）。
对于因圆锥角膜等病症接受DLK的患者，大多数情况下术后细胞密度处于正常水平。因此，通过这种手术干预导致的细胞损失通常较小。这些移植物长期存活的前景良好。
