Harlamert H A, Mira J, Bejarano P A, Baughman R P, Miller M A, Whitsett J A, Yassin R
Department of Pathology and Laboratory Medicine, University of Cincinnati Medical Center, Ohio, USA.
Acta Cytol. 1998 Nov-Dec;42(6):1382-8. doi: 10.1159/000332172.
To evaluate the immunohistochemical expression of a lung epithelial gene transcription factor, thyroid transcription factor-1 (TTF-1), in lung and breast carcinoma in pulmonary cytologic preparations and to correlate the results with the expression of cytokeratin 7 (CK7) and 20 (CK20).
Cell blocks of cytologic specimens were immunostained with antibodies to TTF-1, CK7 and CK20. Specimens included 41 primary lung carcinomas (21 adenocarcinomas, 8 squamous cell carcinomas and 12 small cell undifferentiated carcinomas) and 6 metastatic breast adenocarcinomas.
The lung adenocarcinomas showed nuclear reactivity for TTF-1 in 76% (16/21) of the cases and a staining combination of CK7+/CK20- in 95% (20/21) of the cases. Only one case was CK7+/CK20+. All the breast carcinomas were nonreactive to TTF-1, and all were CK7+/CK20-. The squamous cell carcinomas and small cell undifferentiated carcinomas showed TTF-1 positivity in 38% (3/8) and 83% (10/12), respectively.
评估肺上皮基因转录因子甲状腺转录因子-1（TTF-1）在肺细胞学标本中的肺癌及乳腺癌组织中的免疫组化表达情况，并将结果与细胞角蛋白7（CK7）和20（CK20）的表达进行相关性分析。
对细胞学标本的细胞块进行TTF-1、CK7和CK20抗体免疫染色。标本包括41例原发性肺癌（21例腺癌、8例鳞状细胞癌和12例小细胞未分化癌）和6例转移性乳腺腺癌。
肺腺癌中76%（16/21）的病例TTF-1呈核阳性反应，95%（20/21）的病例为CK7+/CK20-染色组合。仅1例为CK7+/CK20+。所有乳腺癌对TTF-1均无反应，且均为CK7+/CK20-。鳞状细胞癌和小细胞未分化癌中TTF-1阳性率分别为38%（3/8）和83%（10/12）。
