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[绵羊卵母细胞中蛋白质和RNA浓度的年龄及动态变化]

[Age and dynamics of protein and RNA concentration in sheep oocytes].

作者信息

Murzamadiev A M

出版信息

Arkh Anat Gistol Embriol. 1976 Aug;71(8):77-80.

PMID:985101
Abstract

There exists a definite age rhythm of the amount of RNA and protein in Graaf's vesicle oocytes in the postnatal life of the sheep. The lowest quantities of these components were found in the prepubertal lambs; maximum was recorded in 1,5-2,5 year ewes and later the amount of these components decreased and the oocyte of 8,5 year ewes contained 24% less general protein and 69% less RNA than 1,5-2,5 year ewes. As the oocyte grows the amounts of the protein and RNA change. A primordial follicle oocyte contains 250 +/- 15 rel. units of general protein while a Graff's follicle oocytes has three times more (767 +/- 67 rel. unit). The corresponding data for the cytoplasm RNA are: 87,5 +/- 5 rel. units and 163 +/- 18 rel. units which means a 2 times increase.

摘要

绵羊出生后的生命过程中,格拉夫卵泡卵母细胞中的RNA和蛋白质含量存在明确的年龄节律。这些成分的最低含量在青春期前的羔羊中被发现;在1.5至2.5岁的母羊中记录到最大值,之后这些成分的含量下降,8.5岁母羊的卵母细胞所含总蛋白比1.5至2.5岁母羊少24%,RNA少69%。随着卵母细胞的生长,蛋白质和RNA的含量会发生变化。原始卵泡卵母细胞含有250±15相对单位的总蛋白,而格拉夫卵泡卵母细胞的总蛋白含量则是其3倍(767±67相对单位)。细胞质RNA的相应数据为:87.5±5相对单位和163±18相对单位,即增加了2倍。

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