Moreau E, Chauvin A, Boulard C
Ecole nationale vétérinaire de Nantes, France.
Vet Res. 1998 Nov-Dec;29(6):585-91.
Anti-Fasciola hepatica excretory-secretory products (FhESP) and anti-glutathione S-transferase (GST) immune response were studied in sheep infected with 250 F. hepatica metacercariae. In FhESP, 29 proteins with molecular masses (m) of 10-200 kDa were identified by SDS-PAGE. GST m were 26.7 and 27.2 kDa. The anti-FhESP humoral response was precocious and increased throughout the infection. FhESP contains several immunoreactive antigens but no anti-GST antibodies were detected either by ELISA or western blotting. The anti-FhESP cellular response increased precociously and fell after the second week of infection but no cellular response against GST was observed.
对感染250只肝片吸虫囊蚴的绵羊的抗肝片吸虫排泄-分泌产物（FhESP）和抗谷胱甘肽S-转移酶（GST）免疫反应进行了研究。在FhESP中，通过SDS-PAGE鉴定出29种分子量（m）为10-200 kDa的蛋白质。GST的分子量为26.7和27.2 kDa。抗FhESP体液反应出现较早，且在整个感染过程中增强。FhESP含有几种免疫反应性抗原，但通过ELISA或western印迹均未检测到抗GST抗体。抗FhESP细胞反应较早增强，在感染第二周后下降，但未观察到针对GST的细胞反应。
