Rokhlin O W, Weiner G J, Cohen M B
Department of Pathology, University of Iowa, Iowa City 52242, USA.
Cancer Lett. 1998 Sep 25;131(2):129-36. doi: 10.1016/s0304-3835(98)00150-5.
We created mAb that reacted solely with prostate epithelial cells. The strategy involved immunizing mice with a mixture of six different prostatic carcinoma cell lines and selecting by flow cytometry only those antibodies that bind whole cells. The primary screening was performed using a mixture of all six prostate cell lines used in immunization together with six non-prostate cell lines in the same tube. Antibodies that gave a bimodal pattern of surface staining were selected for further evaluation. The most attractive clone, designated 5E10, produced IgG1 mAb that recognized four of the six prostatic cell lines and did not react with non-prostate tumor cell lines, peripheral blood and bone marrow mononuclear cells and endothelial and bone marrow stromal cells. 5E10 mAb reacted with both benign and malignant prostate in eight of eight histological samples and no reactivity was noted with non-prostate normal tissues. The 5E10 antigen is a transmembrane glycoprotein with a molecular weight of 110 kDa and the epitope recognized by 5E10 is extracellular. Ongoing studies are exploring the nature of this antigen in more depth.
我们制备了仅与前列腺上皮细胞发生反应的单克隆抗体。该策略包括用六种不同的前列腺癌细胞系混合物免疫小鼠，并通过流式细胞术仅选择那些能结合完整细胞的抗体。初次筛选是在同一试管中使用免疫所用的全部六种前列腺细胞系与六种非前列腺细胞系的混合物进行的。选择呈现双峰表面染色模式的抗体进行进一步评估。最具吸引力的克隆，命名为5E10，产生了IgG1单克隆抗体，该抗体识别六种前列腺细胞系中的四种，且不与非前列腺肿瘤细胞系、外周血和骨髓单核细胞以及内皮细胞和骨髓基质细胞发生反应。在八个组织学样本中的八个样本中，5E10单克隆抗体与良性和恶性前列腺组织均发生反应，而与非前列腺正常组织未观察到反应性。5E10抗原是一种分子量为110 kDa的跨膜糖蛋白，5E10识别的表位位于细胞外。正在进行的研究正在更深入地探索这种抗原的性质。
