Göçmen C, Seçilmiş A, Uçar P, Karataş Y, Onder S, Dikmen A, Baysal F
Cukurova University, Medical Faculty, Department of Pharmacology, Adana, Turkey.
Eur J Pharmacol. 1998 Nov 13;361(1):85-92. doi: 10.1016/s0014-2999(98)00703-1.
Relaxations induced by electrical field stimulation and acetylcholine were compared with those induced by acidified sodium nitrite, sodium nitroprusside, S-nitrosoglutathione and S-nitroso-N-acetyl-D,L-penicillamine in the mouse corpus cavernosum precontracted with phenylephrine. NG-nitro-L-arginine inhibited electrical field stimulation- or acetylcholine-induced relaxation, but was ineffective on relaxations caused by the other stimuli. Hydroquinone and pyrogallol had no inhibitory action on the relaxations caused by any stimulus except acidified sodium nitrite. Incubation of the tissue with diethyldithiocarbamic acid significantly inhibited the relaxations induced by all stimuli except papaverine. In the tissues pre-treated with diethyldithiocarbamic acid, superoxide dismutase, hydroquinone and pyrogallol failed to yield restore or further inhibit the relaxations in response to electrical field stimulation or acetylcholine. LY 83583 (6-anilino-5,8-quinolinedione) and hydroxocobalamin clearly inhibited the relaxant responses to electrical field stimulation, acetylcholine, S-nitrosoglutathione and acidified sodium nitrite whereas there was significant enhancement of the relaxation produced by S-nitroso-N-acetyl-D,L-penicillamine. These findings suggest that the relaxant factor released from non-adrenergic non-cholinergic nerves or endothelial cells in mouse cavernosal tissue may be a superoxide anion-resistant nitric oxide-containing molecule and that S-nitrosoglutathione rather than S-nitroso-N-acetyl-D,L-penicillamine could be a suitable candidate for this.
在苯肾上腺素预收缩的小鼠海绵体中，比较了电场刺激和乙酰胆碱诱导的舒张与酸化亚硝酸钠、硝普钠、S-亚硝基谷胱甘肽和S-亚硝基-N-乙酰-D,L-青霉胺诱导的舒张。NG-硝基-L-精氨酸抑制电场刺激或乙酰胆碱诱导的舒张，但对其他刺激引起的舒张无效。对苯二酚和邻苯三酚对除酸化亚硝酸钠外的任何刺激引起的舒张均无抑制作用。用二乙基二硫代氨基甲酸盐孵育组织可显著抑制除罂粟碱外所有刺激诱导的舒张。在用二乙基二硫代氨基甲酸盐预处理的组织中，超氧化物歧化酶、对苯二酚和邻苯三酚未能恢复或进一步抑制对电场刺激或乙酰胆碱的舒张反应。LY 83583（6-苯胺基-5,8-喹啉二酮）和羟钴胺素明显抑制对电场刺激、乙酰胆碱、S-亚硝基谷胱甘肽和酸化亚硝酸钠的舒张反应，而S-亚硝基-N-乙酰-D,L-青霉胺产生的舒张则显著增强。这些发现表明，从小鼠海绵体组织中非肾上腺素能非胆碱能神经或内皮细胞释放的舒张因子可能是一种抗超氧阴离子的含一氧化氮分子，并且S-亚硝基谷胱甘肽而非S-亚硝基-N-乙酰-D,L-青霉胺可能是其合适的候选物。
