大肠杆菌O35 O抗原多糖的结构测定及其与亚利桑那沙门氏菌O62的交叉反应性

Structural determination of the O-antigenic polysaccharide from Escherichia coli O35 and cross-reactivity to Salmonella arizonae O62.

作者信息

Rundlöf T, Weintraub A, Widmalm G

机构信息

Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Sweden.

出版信息

Eur J Biochem. 1998 Nov 15;258(1):139-43. doi: 10.1046/j.1432-1327.1998.2580139.x.

DOI:10.1046/j.1432-1327.1998.2580139.x

PMID:9851702

Abstract

The structure of the O-antigenic polysaccharide from Escherichia coli O35 has been investigated with the aid of NMR spectroscopy, sugar and methylation analyses. The sequence of the sugar residues could be determined by NOESY and heteronuclear-multiple-bond-connectivity NMR experiments. The polysaccharide is composed of hexasaccharide repeating units with the following structure, where Rha and GalNAcAN represent rhamnose and 2-acetamido-2-deoxy-galacturonamide, respectively: carbohydrate sequence [see text]. The O-antigen of Escherichia coli O35 is similar to the O-specific polysaccharide from Salmonella arizonae O62, which instead has a terminal 2-acetamido-2-deoxy-alpha-D-galacturonic acid residue. Immunochemical analyses using a rabbit antiserum specific for the Salmonella arizonae O62 O-antigen showed an identical reactivity with both lipopolysaccharides.

摘要

借助核磁共振光谱、糖类及甲基化分析，对来自大肠杆菌O35的O抗原多糖结构进行了研究。糖残基序列可通过NOESY和异核多键连接核磁共振实验确定。该多糖由具有以下结构的六糖重复单元组成，其中Rha和GalNAcAN分别代表鼠李糖和2-乙酰氨基-2-脱氧半乳糖醛酰胺：碳水化合物序列[见正文]。大肠杆菌O35的O抗原与亚利桑那沙门氏菌O62的O特异性多糖相似，后者取而代之的是具有一个末端2-乙酰氨基-2-脱氧-α-D-半乳糖醛酸残基。使用针对亚利桑那沙门氏菌O62 O抗原的兔抗血清进行的免疫化学分析表明，两种脂多糖具有相同的反应性。