Hwa V, Tomasini-Sprenger C, Bermejo A L, Rosenfeld R G, Plymate S R
Department of Pediatrics, Oregon Health Sciences University, Portland 97201, USA.
J Clin Endocrinol Metab. 1998 Dec;83(12):4355-62. doi: 10.1210/jcem.83.12.5341.
Insulin-like growth factor-binding protein-related protein-1 (IGFBP-rP1; also known as Mac25, TAF, and PSF) is a member of the IGFBP superfamily. It is a cysteine-rich protein that shares structural and functional similarities with the conventional IGFBPs. In situ hybridization of prostate tissue sections show intense IGFBP-rP1 messenger ribonucleic acid (mRNA) expression in normal stroma and glandular epithelium. There was a significant loss of detectable IGFBP-rP1 mRNA in metastatic prostate tissue. IGFBP-rP1 mRNA (Northern blots) and protein (immunoblots) were detectable in primary cultures ofprostatic stromal and epithelial cells as well as in the immortalized nonmalignant prostatic human epithelial cells, P69, and in the P69 metastatic subline, M12. IGFBP-rP1 expression was not detectable in the prostatic cancer cell lines PC-3, DU145, and LNCaP. IGFBP-rP1 expression was regulated in P69 cells but not in M12 cells. Protein and mRNA expression was up-regulated by IGF-I, transforming growth factor-beta, and retinoic acid. The observations that IGFBP-rP1 expression is significantly diminished in prostate tumorigenesis and is regulated in nonmalignant prostate cells suggest IGFBP-rP1 is important in normal prostatic cell growth.
胰岛素样生长因子结合蛋白相关蛋白-1（IGFBP-rP1；也称为Mac25、TAF和PSF）是IGFBP超家族的成员。它是一种富含半胱氨酸的蛋白质，与传统的IGFBPs在结构和功能上具有相似性。前列腺组织切片的原位杂交显示，在正常基质和腺上皮中IGFBP-rP1信使核糖核酸（mRNA）表达强烈。在转移性前列腺组织中，可检测到的IGFBP-rP1 mRNA显著减少。在前列腺基质和上皮细胞的原代培养物中，以及在永生化的非恶性人前列腺上皮细胞P69及其转移性亚系M12中，均可检测到IGFBP-rP1 mRNA（Northern印迹法）和蛋白质（免疫印迹法）。在前列腺癌细胞系PC-3、DU145和LNCaP中未检测到IGFBP-rP1表达。IGFBP-rP1表达在P69细胞中受到调节，但在M12细胞中不受调节。蛋白质和mRNA表达受IGF-I、转化生长因子-β和视黄酸上调。IGFBP-rP1表达在前列腺肿瘤发生过程中显著降低且在非恶性前列腺细胞中受到调节，这些观察结果表明IGFBP-rP1在正常前列腺细胞生长中很重要。
