果蝇细胞周期蛋白B3是雌性生育所必需的，并且像细胞周期蛋白B一样，对有丝分裂是可有可无的。
Drosophila Cyclin B3 is required for female fertility and is dispensable for mitosis like Cyclin B.
作者信息
Jacobs H W, Knoblich J A, Lehner C F
机构信息
Department of Genetics, University of Bayreuth, 95440 Bayreuth, Germany.
出版信息
Genes Dev. 1998 Dec 1;12(23):3741-51. doi: 10.1101/gad.12.23.3741.
Abstract
Cyclin B3 has been conserved during higher eukaryote evolution as evidenced by its identification in chicken, nematodes, and insects. We demonstrate that Cyclin B3 is present in addition to Cyclins A and B in mitotically proliferating cells and not detectable in endoreduplicating tissues of Drosophila embryos. Cyclin B3 is coimmunoprecipitated with Cdk1(Cdc2) but not with Cdk2(Cdc2c). It is degraded abruptly during mitosis like Cyclins A and B. In contrast to these latter cyclins, which accumulate predominantly in the cytoplasm during interphase, Cyclin B3 is a nuclear protein. Genetic analyses indicate functional redundancies. Double and triple mutant analyses demonstrate that Cyclins A, B, and B3 cooperate to regulate mitosis, but surprisingly single mutants reveal that neither Cyclin B3 nor Cyclin B is required for mitosis. However, both are required for female fertility and Cyclin B also for male fertility.
摘要
细胞周期蛋白B3在高等真核生物进化过程中一直保守，这在鸡、线虫和昆虫中均有发现可资证明。我们证明，在有丝分裂增殖细胞中，除了细胞周期蛋白A和B之外，细胞周期蛋白B3也存在，而在果蝇胚胎的核内复制组织中则检测不到。细胞周期蛋白B3与Cdk1（Cdc2）共免疫沉淀，但不与Cdk2（Cdc2c）共免疫沉淀。它在有丝分裂期间像细胞周期蛋白A和B一样突然降解。与后两种主要在间期积聚在细胞质中的细胞周期蛋白不同，细胞周期蛋白B3是一种核蛋白。遗传分析表明存在功能冗余。双突变和三突变分析表明，细胞周期蛋白A、B和B3协同调节有丝分裂，但令人惊讶的是，单突变表明有丝分裂既不需要细胞周期蛋白B3也不需要细胞周期蛋白B。然而，二者对于雌性生育力都是必需的，细胞周期蛋白B对雄性生育力也是必需的。
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