大肠杆菌钠偶联丝氨酸转运蛋白基因的克隆、表达及该转运蛋白的特性
Cloning and expression of the gene for the Na+-coupled serine transporter from Escherichia coli and characteristics of the transporter.
作者信息
Ogawa W, Kim Y M, Mizushima T, Tsuchiya T
机构信息
Department of Microbiology, Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama 700-8530, Japan.
出版信息
J Bacteriol. 1998 Dec;180(24):6749-52. doi: 10.1128/JB.180.24.6749-6752.1998.
Abstract
We cloned a gene (sstT) for the Na+/serine symporter from the chromosome of Escherichia coli by using a low-copy-number vector and sequenced it. According to the deduced amino acid sequence, the transporter (SstT) consists of 414 amino acid residues. Hydropathy analysis suggested that the SstT protein possesses 9, instead of 12, hydrophobic domains.
摘要
我们使用低拷贝数载体从大肠杆菌染色体中克隆了一个编码Na⁺/丝氨酸同向转运体的基因（sstT）并对其进行了测序。根据推导的氨基酸序列，该转运体（SstT）由414个氨基酸残基组成。亲水性分析表明，SstT蛋白具有9个而非12个疏水结构域。
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