• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Cloning and expression of the gene for the Na+-coupled serine transporter from Escherichia coli and characteristics of the transporter.大肠杆菌钠偶联丝氨酸转运蛋白基因的克隆、表达及该转运蛋白的特性
J Bacteriol. 1998 Dec;180(24):6749-52. doi: 10.1128/JB.180.24.6749-6752.1998.
2
Purification, reconstitution, and characterization of Na(+)/serine symporter, SstT, of Escherichia coli.大肠杆菌钠/丝氨酸同向转运体SstT的纯化、重组及特性分析
J Biochem. 2002 Jul;132(1):71-6. doi: 10.1093/oxfordjournals.jbchem.a003201.
3
Isolation and characterization of an Escherichia coli mutant lacking the major serine transporter, and cloning of a serine transporter gene.一株缺乏主要丝氨酸转运蛋白的大肠杆菌突变体的分离与鉴定，以及丝氨酸转运蛋白基因的克隆
J Biochem. 1997 Dec;122(6):1241-5. doi: 10.1093/oxfordjournals.jbchem.a021887.
4
Sodium ion-driven serine/threonine transport in Porphyromonas gingivalis.牙龈卟啉单胞菌中钠离子驱动的丝氨酸/苏氨酸转运
J Bacteriol. 2001 Jul;183(14):4142-8. doi: 10.1128/JB.183.14.4142-4148.2001.
5
The small RNA GcvB regulates sstT mRNA expression in Escherichia coli.小RNA GcvB在大肠杆菌中调节sstT mRNA的表达。
J Bacteriol. 2009 Jan;191(1):238-48. doi: 10.1128/JB.00915-08. Epub 2008 Oct 24.
6
Role of Ser-340 and Thr-341 in transmembrane domain IX of the Na+/proline transporter PutP of Escherichia coli in ligand binding and transport.大肠杆菌Na⁺/脯氨酸转运蛋白PutP跨膜结构域IX中Ser-340和Thr-341在配体结合和转运中的作用
J Biol Chem. 2008 Feb 22;283(8):4921-9. doi: 10.1074/jbc.M706741200. Epub 2007 Dec 21.
7
Properties of a Na+-coupled serine-threonine transport system in Escherichia coli.大肠杆菌中一种钠离子偶联的丝氨酸-苏氨酸转运系统的特性
Biochim Biophys Acta. 1987 Dec 11;905(2):231-9. doi: 10.1016/0005-2736(87)90451-2.
8
Expression cloning of a Na+-independent aromatic amino acid transporter with structural similarity to H+/monocarboxylate transporters.一种与H⁺/单羧酸转运蛋白结构相似的非Na⁺依赖性芳香族氨基酸转运蛋白的表达克隆。
J Biol Chem. 2001 May 18;276(20):17221-8. doi: 10.1074/jbc.M009462200. Epub 2001 Feb 20.
9
Identification and sequence of a Na(+)-linked gene from the marine bacterium Alteromonas haloplanktis which functionally complements the dagA gene of Escherichia coli.
Mol Microbiol. 1992 Sep;6(18):2673-81. doi: 10.1111/j.1365-2958.1992.tb01444.x.
10
Cloning and sequencing of the pheP gene, which encodes the phenylalanine-specific transport system of Escherichia coli.编码大肠杆菌苯丙氨酸特异性转运系统的pheP基因的克隆与测序。
J Bacteriol. 1991 Jun;173(12):3622-9. doi: 10.1128/jb.173.12.3622-3629.1991.

引用本文的文献

1
Bioprocess Engineering, Transcriptome, and Intermediate Metabolite Analysis of L-Serine High-Yielding W3110.L-丝氨酸高产菌株W3110的生物过程工程、转录组及中间代谢物分析
Microorganisms. 2022 Sep 28;10(10):1927. doi: 10.3390/microorganisms10101927.
2
The Conserved Serine Transporter SdaC Moonlights To Enable Self Recognition.保守丝氨酸转运蛋白 SdaC 兼职以实现自我识别。
J Bacteriol. 2022 Jan 18;204(1):e0034721. doi: 10.1128/JB.00347-21. Epub 2021 Oct 18.
3
Genomic plasticity of pathogenic mediates d-serine tolerance via multiple adaptive mechanisms.致病微生物的基因组可塑性通过多种适应性机制介导 D-丝氨酸耐受性。
Proc Natl Acad Sci U S A. 2020 Sep 8;117(36):22484-22493. doi: 10.1073/pnas.2004977117. Epub 2020 Aug 26.
4
Resistance to serine in Bacillus subtilis: identification of the serine transporter YbeC and of a metabolic network that links serine and threonine metabolism.枯草芽孢杆菌中丝氨酸的抗性：丝氨酸转运蛋白 YbeC 的鉴定和丝氨酸与苏氨酸代谢相联系的代谢网络。
Environ Microbiol. 2020 Sep;22(9):3937-3949. doi: 10.1111/1462-2920.15179. Epub 2020 Aug 13.
5
The serine transporter SdaC prevents cell lysis upon glucose depletion in Escherichia coli.丝氨酸转运蛋白 SdaC 可防止大肠杆菌在葡萄糖耗尽时发生细胞裂解。
Microbiologyopen. 2020 Feb;9(2):e960. doi: 10.1002/mbo3.960. Epub 2019 Nov 3.
6
Characterization of MATE-type multidrug efflux pumps from Klebsiella pneumoniae MGH78578.肺炎克雷伯菌MGH78578中MATE型多药外排泵的特性分析
PLoS One. 2015 Mar 25;10(3):e0121619. doi: 10.1371/journal.pone.0121619. eCollection 2015.
7
Functionally cloned pdrM from Streptococcus pneumoniae encodes a Na(+) coupled multidrug efflux pump.功能克隆的肺炎链球菌 pdrM 编码一种 Na(+) 偶联的多药外排泵。
PLoS One. 2013;8(3):e59525. doi: 10.1371/journal.pone.0059525. Epub 2013 Mar 26.
8
The small RNA GcvB regulates sstT mRNA expression in Escherichia coli.小RNA GcvB在大肠杆菌中调节sstT mRNA的表达。
J Bacteriol. 2009 Jan;191(1):238-48. doi: 10.1128/JB.00915-08. Epub 2008 Oct 24.
9
Transcriptome analysis of the ArgR regulon in Pseudomonas aeruginosa.铜绿假单胞菌中ArgR调控子的转录组分析。
J Bacteriol. 2004 Jun;186(12):3855-61. doi: 10.1128/JB.186.12.3855-3861.2004.
10
Dynamic diversity of the tryptophan pathway in chlamydiae: reductive evolution and a novel operon for tryptophan recapture.衣原体中色氨酸途径的动态多样性：还原进化与一个用于色氨酸重新捕获的新操纵子。
Genome Biol. 2002 Aug 29;3(9):research0051. doi: 10.1186/gb-2002-3-9-research0051.

本文引用的文献

1
ISOLATION OF HIGH MOLECULAR WEIGHT DNA FROM HEMOPHILUS INFLUENZAE.从流感嗜血杆菌中分离高分子量DNA。
J Mol Biol. 1965 Mar;11:476-90. doi: 10.1016/s0022-2836(65)80004-3.
2
Isolation and characterization of an Escherichia coli mutant lacking the major serine transporter, and cloning of a serine transporter gene.一株缺乏主要丝氨酸转运蛋白的大肠杆菌突变体的分离与鉴定，以及丝氨酸转运蛋白基因的克隆
J Biochem. 1997 Dec;122(6):1241-5. doi: 10.1093/oxfordjournals.jbchem.a021887.
3
The complete genome sequence of Escherichia coli K-12.大肠杆菌K-12的全基因组序列。
Science. 1997 Sep 5;277(5331):1453-62. doi: 10.1126/science.277.5331.1453.
4
Structure and function of Escherichia coli met repressor: similarities and contrasts with trp repressor.
Philos Trans R Soc Lond B Biol Sci. 1996 Apr 29;351(1339):527-35. doi: 10.1098/rstb.1996.0051.
5
Cloning and functional characterization of a system ASC-like Na+-dependent neutral amino acid transporter.一种类ASC系统的钠离子依赖性中性氨基酸转运体的克隆与功能表征
J Biol Chem. 1996 Jun 21;271(25):14883-90. doi: 10.1074/jbc.271.25.14883.
6
Isolation and sequencing of Escherichia coli gene proP reveals unusual structural features of the osmoregulatory proline/betaine transporter, ProP.大肠杆菌基因proP的分离与测序揭示了渗透调节性脯氨酸/甜菜碱转运蛋白ProP不同寻常的结构特征。
J Mol Biol. 1993 Jan 5;229(1):268-76. doi: 10.1006/jmbi.1993.1030.
7
Cloning and expression of a novel Na(+)-dependent neutral amino acid transporter structurally related to mammalian Na+/glutamate cotransporters.一种与哺乳动物钠/谷氨酸共转运体结构相关的新型钠依赖性中性氨基酸转运体的克隆与表达。
J Biol Chem. 1993 Jul 25;268(21):15351-5.
8
Molecular organization of the Escherichia coli gab cluster: nucleotide sequence of the structural genes gabD and gabP and expression of the GABA permease gene.大肠杆菌gab操纵子的分子组织：结构基因gabD和gabP的核苷酸序列以及GABA通透酶基因的表达
Arch Microbiol. 1993;160(6):454-60. doi: 10.1007/BF00245306.
9
Cloning and expression of a human neutral amino acid transporter with structural similarity to the glutamate transporter gene family.一种与谷氨酸转运体基因家族结构相似的人类中性氨基酸转运体的克隆与表达。
J Biol Chem. 1993 Jul 25;268(21):15329-32.
10
Sequencing and characterization of the sdaC gene and identification of the sdaCB operon in Escherichia coli K12.大肠杆菌K12中sdaC基因的测序与特征分析及sdaCB操纵子的鉴定
Eur J Biochem. 1994 Jun 15;222(3):901-7. doi: 10.1111/j.1432-1033.1994.tb18938.x.

大肠杆菌钠偶联丝氨酸转运蛋白基因的克隆、表达及该转运蛋白的特性

Cloning and expression of the gene for the Na+-coupled serine transporter from Escherichia coli and characteristics of the transporter.

作者信息

Ogawa W, Kim Y M, Mizushima T, Tsuchiya T

机构信息

Department of Microbiology, Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama 700-8530, Japan.

出版信息

J Bacteriol. 1998 Dec;180(24):6749-52. doi: 10.1128/JB.180.24.6749-6752.1998.

DOI:10.1128/JB.180.24.6749-6752.1998
PMID:9852024
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC107783/
Abstract

We cloned a gene (sstT) for the Na+/serine symporter from the chromosome of Escherichia coli by using a low-copy-number vector and sequenced it. According to the deduced amino acid sequence, the transporter (SstT) consists of 414 amino acid residues. Hydropathy analysis suggested that the SstT protein possesses 9, instead of 12, hydrophobic domains.

摘要

我们使用低拷贝数载体从大肠杆菌染色体中克隆了一个编码Na⁺/丝氨酸同向转运体的基因（sstT）并对其进行了测序。根据推导的氨基酸序列，该转运体（SstT）由414个氨基酸残基组成。亲水性分析表明，SstT蛋白具有9个而非12个疏水结构域。