恶臭假单胞菌S12对有机溶剂的主动外排是由溶剂诱导的。
Active efflux of organic solvents by Pseudomonas putida S12 is induced by solvents.
作者信息
Kieboom J, Dennis J J, Zylstra G J, de Bont J A
机构信息
Division of Industrial Microbiology, Department of Food Technology and Nutritional Sciences, Wageningen Agricultural University, Wageningen, The Netherlands.
出版信息
J Bacteriol. 1998 Dec;180(24):6769-72. doi: 10.1128/JB.180.24.6769-6772.1998.
Abstract
Induction of the membrane-associated organic solvent efflux system SrpABC of Pseudomonas putida S12 was examined by cloning a 312-bp DNA fragment, containing the srp promoter, in the broad-host-range reporter vector pKRZ-1. Compounds that are capable of inducing expression of the srpABC genes include aromatic and aliphatic solvents and alcohols. General stress conditions such as pH, temperature, NaCl, or the presence of organic acids did not induce srp transcription. Although the solvent efflux pump in P. putida S12 is a member of the resistance-nodulation-cell division family of transporters, the srpABC genes were not induced by antibiotics or heavy metals.
摘要
通过将包含srp启动子的312 bp DNA片段克隆到广宿主范围报告载体pKRZ - 1中，研究了恶臭假单胞菌S12膜相关有机溶剂外排系统SrpABC的诱导情况。能够诱导srpABC基因表达的化合物包括芳香族和脂肪族溶剂以及醇类。一般应激条件如pH、温度、NaCl或有机酸的存在均不会诱导srp转录。尽管恶臭假单胞菌S12中的溶剂外排泵是转运蛋白抗药 - 结瘤 - 细胞分裂家族的成员，但srpABC基因不会被抗生素或重金属诱导。
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