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本文引用的文献

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Effect of Environmental Factors on the trans/cis Ratio of Unsaturated Fatty Acids in Pseudomonas putida S12.环境因素对铜绿假单胞菌 S12 中不饱和脂肪酸顺/反式比例的影响。
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Cell Envelope Changes in Solvent-Tolerant and Solvent-Sensitive Pseudomonas putida Strains following Exposure to o-Xylene.溶剂耐受型和敏感型铜绿假单胞菌菌株暴露于间二甲苯后细胞包膜的变化。
Appl Environ Microbiol. 1996 Mar;62(3):1129-32. doi: 10.1128/aem.62.3.1129-1132.1996.
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Metabolism of Styrene Oxide and 2-Phenylethanol in the Styrene-Degrading Xanthobacter Strain 124X.苯乙烯氧化物和 2-苯乙醇在苯乙烯降解黄杆菌 124X 中的代谢。
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Isolation and transposon mutagenesis of a Pseudomonas putida KT2442 toluene-resistant variant: involvement of an efflux system in solvent resistance.恶臭假单胞菌KT2442耐甲苯变体的分离及转座子诱变：一种外排系统在溶剂抗性中的作用
Extremophiles. 1998 Nov;2(4):395-400. doi: 10.1007/s007920050084.
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Isolation and characterization of toluene-sensitive mutants from the toluene-resistant bacterium Pseudomonas putida GM73.从抗甲苯细菌恶臭假单胞菌GM73中分离和鉴定甲苯敏感突变体。
J Bacteriol. 1998 Jul;180(14):3692-6. doi: 10.1128/JB.180.14.3692-3696.1998.
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Efflux pumps involved in toluene tolerance in Pseudomonas putida DOT-T1E.恶臭假单胞菌DOT-T1E中参与甲苯耐受性的外排泵
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Role of the multidrug efflux systems of Pseudomonas aeruginosa in organic solvent tolerance.铜绿假单胞菌多药外排系统在有机溶剂耐受性中的作用。
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Identification and molecular characterization of an efflux pump involved in Pseudomonas putida S12 solvent tolerance.恶臭假单胞菌S12溶剂耐受性相关外排泵的鉴定及分子特征分析
J Biol Chem. 1998 Jan 2;273(1):85-91. doi: 10.1074/jbc.273.1.85.
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恶臭假单胞菌S12对有机溶剂的主动外排是由溶剂诱导的。

Active efflux of organic solvents by Pseudomonas putida S12 is induced by solvents.

作者信息

Kieboom J, Dennis J J, Zylstra G J, de Bont J A

机构信息

Division of Industrial Microbiology, Department of Food Technology and Nutritional Sciences, Wageningen Agricultural University, Wageningen, The Netherlands.

出版信息

J Bacteriol. 1998 Dec;180(24):6769-72. doi: 10.1128/JB.180.24.6769-6772.1998.

DOI:10.1128/JB.180.24.6769-6772.1998
PMID:9852029
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC107788/
Abstract

Induction of the membrane-associated organic solvent efflux system SrpABC of Pseudomonas putida S12 was examined by cloning a 312-bp DNA fragment, containing the srp promoter, in the broad-host-range reporter vector pKRZ-1. Compounds that are capable of inducing expression of the srpABC genes include aromatic and aliphatic solvents and alcohols. General stress conditions such as pH, temperature, NaCl, or the presence of organic acids did not induce srp transcription. Although the solvent efflux pump in P. putida S12 is a member of the resistance-nodulation-cell division family of transporters, the srpABC genes were not induced by antibiotics or heavy metals.

摘要

通过将包含srp启动子的312 bp DNA片段克隆到广宿主范围报告载体pKRZ - 1中，研究了恶臭假单胞菌S12膜相关有机溶剂外排系统SrpABC的诱导情况。能够诱导srpABC基因表达的化合物包括芳香族和脂肪族溶剂以及醇类。一般应激条件如pH、温度、NaCl或有机酸的存在均不会诱导srp转录。尽管恶臭假单胞菌S12中的溶剂外排泵是转运蛋白抗药 - 结瘤 - 细胞分裂家族的成员，但srpABC基因不会被抗生素或重金属诱导。