Judson G J, Filsell O H, Jarrett I G
Aust J Biol Sci. 1976 Jul;29(3):215-22. doi: 10.1071/bi9760215.
Total entry rate of blood glucose and the rate of irreversible loss of blood acetate and its oxidation have been examined in sheep at rest and while walking on a horizontal treadmill at 5 km/h for 2 h. Sheep were given their daily ration of 1000 g chaff in 24 eaual portions at hourly intervals and received multiple intravenous injections of [2-3H]glucose and intravenous infusions of [1-14C]acetate and NaH14CO3. At rest the total entry rate of blood glucose was 0-44 +/- 0-03 mmol/min (values given as mean +/- s.e.m. for four sheep), the glucose pool was 23 +/- 1 mmol and the rate of irreversible loss of blood acetate was 2-3 +/- 0-1 mmol/min. During exercise, the total entry rate of blood glucose was 0-84 +/- 0-04 mmol/min, the glucose pool was 27 +/- 2 mmol and the rate of irreversible loss of blood acetate was 2-6 +/- 0-1 mmol/min. Gluconeogenesis apparently increased markedly in response to exercise as indicated by the incorporation of 14C from blood bicarbonate into blood glucose. Despite the substantial increase in the rate of irreversible loss of blood bicarbonate (from 11-6 +/- 1 to 20-2 +/- 2 mmol C/min), and hence energy expenditure with exercise, only a slight change was recorded in the proportion of the irreversible loss rate of acetate that was oxidized.
已对处于静息状态以及在水平跑步机上以5公里/小时的速度行走2小时的绵羊，检测了血糖的总进入速率以及血液中乙酸盐的不可逆损失速率及其氧化速率。绵羊每小时以24等份的形式给予其每日定量的1000克谷糠，并接受多次静脉注射[2-³H]葡萄糖以及静脉输注[1-¹⁴C]乙酸盐和NaH¹⁴CO₃。静息时，血糖的总进入速率为0.44±0.03毫摩尔/分钟（数值为4只绵羊的平均值±标准误），葡萄糖池为23±1毫摩尔，血液中乙酸盐的不可逆损失速率为2.3±0.1毫摩尔/分钟。运动期间，血糖的总进入速率为0.84±0.04毫摩尔/分钟，葡萄糖池为27±2毫摩尔，血液中乙酸盐的不可逆损失速率为2.6±0.1毫摩尔/分钟。如血液碳酸氢盐中的¹⁴C掺入血糖所示，糖异生明显因运动而显著增加。尽管血液碳酸氢盐的不可逆损失速率大幅增加（从11.6±1至20.2±2毫摩尔碳/分钟），因此运动时能量消耗增加，但乙酸盐不可逆损失速率中被氧化部分的比例仅记录到轻微变化。
