Cho Y, Lee I, Maul G G, Yu E
Department of Pathology, University of Ulsan College of Medicine, Songpa-gu, Seoul 138-736, Korea.
Int J Mol Med. 1998 Apr;1(4):717-24. doi: 10.3892/ijmm.1.4.717.
ND10 are recently characterized nuclear domains that are composed of 0.5 microm sized, precisely circumscribed dots in cultured human cell lines. To investigate the distribution and number of ND10 on various types of normal and neoplastic human tissues, we carried out immunostaining and immunoprecipitation analyses with monoclonal antibodies 138 and 1150. The number of ND10 varied from 1 to 10 or more in various tissues as did their size. ND10 were diffusely located in early embryonic and normal tissues, except for the exocrine and endocrine cells of the pancreas and for hepatocytes. In normal squamous mucosa, basal cells had more ND10 than did differentiated superficial squamous cells. The number and size of ND10 were markedly increased in malignant neoplasms but were similar in benign tumors and corresponding normal tissues. Sex hormone-related normal tissues, such as the endometrium or myometrium, and neoplasms strongly stained for ND10. The distribution pattern of ND10 in human tissues indicates that they are conserved nuclear substructures that are closely associated with cellular differentiation, hormonal stimulation, and oncogenesis.
ND10是最近鉴定出的核结构域，在培养的人类细胞系中，它由大小为0.5微米、边界精确的点状物组成。为了研究ND10在各种正常和肿瘤性人类组织中的分布及数量，我们用单克隆抗体138和1150进行了免疫染色和免疫沉淀分析。ND10的数量在不同组织中从1到10个或更多不等，其大小也是如此。ND10广泛分布于早期胚胎组织和正常组织中，但胰腺的外分泌和内分泌细胞以及肝细胞除外。在正常鳞状黏膜中，基底细胞的ND10比分化的表层鳞状细胞更多。恶性肿瘤中ND10的数量和大小显著增加，但良性肿瘤和相应正常组织中的情况相似。与性激素相关的正常组织，如子宫内膜或子宫肌层，以及肿瘤对ND10呈强染色。ND10在人类组织中的分布模式表明，它们是保守的核亚结构，与细胞分化、激素刺激和肿瘤发生密切相关。
