鸡绒毛尿囊膜的钙结合蛋白。I. 免疫组织化学定位
Calcium-binding protein of the chick chorioallantoic membrane. I. Immunohistochemical localization.
作者信息
Tuan R S, Scott W A, Cohn Z A
出版信息
J Cell Biol. 1978 Jun;77(3):743-51. doi: 10.1083/jcb.77.3.743.
Abstract
The preparation of a specific antiserum (anti-CaBP) against the calcium-binding protein (CaBP) of the chorioallantoic membrane (CAM) is described. The anti-CaBP appeared to be specific for the CaBP by immunodiffusion and immunoelectrophoresis. Application of the anti-CaBP in immunofluorescence histochemistry revealed that the CaBP is present in the CAM only at developmental ages corresponding with the expression of the calcium transport function of the membrane. Furthermore, the CaBP is localized to the ectoderm of the CAM, appears to be exposed to the entire external surface of the ectoderm, and can be shown to be associated with cells enzymatically dissociated from the CAM. These results are consistent with a functional role of the CaBP in the CAM calcium transport process.
摘要
本文描述了针对绒毛尿囊膜（CAM）钙结合蛋白（CaBP）制备特异性抗血清（抗CaBP）的过程。通过免疫扩散和免疫电泳，抗CaBP似乎对CaBP具有特异性。抗CaBP在免疫荧光组织化学中的应用表明，CaBP仅在与该膜钙转运功能表达相对应的发育阶段存在于CAM中。此外，CaBP定位于CAM的外胚层，似乎暴露于外胚层的整个外表面，并且可以证明其与从CAM酶解分离的细胞相关。这些结果与CaBP在CAM钙转运过程中的功能作用一致。
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