Sakata H, Kurita M, Murakami Y, Nagasawa S, Watanabe M, Ueda S, Matsumoto M, Sato T, Kobune F, Seya T
Department of Virus Disease and Vaccine Control, National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan.
Biol Pharm Bull. 1998 Nov;21(11):1121-7. doi: 10.1248/bpb.21.1121.
Down-regulation of CD46 secondary to stimulation with measles virus (MV) was investigated using CD46-positive cell lines and Japanese wild-type MV strains. The cells used were simian cell lines B95a and Vero in which MV strains have been adapted to be amplified, and Chinese hamster ovary (CHO) cell transfectants expressing human CD46 with (CHO(tail+)) or without (CHO(tail-)) the cytoplasmic tail. Of four Vero-adapted and three B95a-adapted MV strains, one Vero-adapted strain named Khono (KO), down-regulated CD46 within 60 min (early down-regulation) in all cell lines examined except Vero. No strains other than Toyoshima (TY), which induced early down-regulation only in CHO(tail+) cells, induced early down-regulation of CD46 in any combination. On the other hand, conventional down-regulation of CD46 was observed 24 h post-MV inoculation (late down-regulation) when cell lines used were adapted to MV strains. Thus, we concluded that there are two modes of CD46 down-regulation by MV and the unique strain KO markedly induces early down-regulation. Also, the CD46 homologue of B95a, which fails to act as a MV receptor, is down-regulated concomitantly with MV replication (>24 h) in cells principally by competent virus strains.
利用CD46阳性细胞系和日本野生型麻疹病毒（MV）株，研究了MV刺激后CD46的下调情况。所使用的细胞为已适应于扩增MV株的猿猴细胞系B95a和Vero，以及表达人CD46且带有（CHO(tail+)）或不带有（CHO(tail-)）细胞质尾的中国仓鼠卵巢（CHO）细胞转染体。在4株适应于Vero的MV株和3株适应于B95a的MV株中，1株名为Khono（KO）的适应于Vero的毒株，在60分钟内（早期下调）使除Vero外的所有检测细胞系中的CD46下调。除仅在CHO(tail+)细胞中诱导早期下调的丰岛（TY）外，没有其他毒株能以任何组合诱导CD46的早期下调。另一方面，当所用细胞系适应于MV株时，在接种MV后24小时观察到CD46的常规下调（晚期下调）。因此，我们得出结论，MV对CD46的下调有两种模式，独特的KO毒株显著诱导早期下调。此外，不能作为MV受体的B95a的CD46同源物，在细胞中主要由有活性的病毒株在MV复制（>24小时）时伴随下调。
