Schneider-Schaulies J, Schnorr J J, Brinckmann U, Dunster L M, Baczko K, Liebert U G, Schneider-Schaulies S, ter Meulen V
Institut für Virologie und Immunbiologie, W urzburg, Germany.
Proc Natl Acad Sci U S A. 1995 Apr 25;92(9):3943-7. doi: 10.1073/pnas.92.9.3943.
Recently, two cell surface molecules, CD46 and moesin, have been found to be functionally associated with measles virus (MV) infectivity of cells. We investigated the receptor usage of MV wild-type, subacute sclerosing panencephalitis, and vaccine strains and their effect on the down-regulation of CD46 after infection. We found that the infection of human cell lines with all 19 MV strains tested was inhibitable with antibodies against CD46. In contrast, not all strains of MV led to the downregulation of CD46 following infection. The group of CD46 non-downregulating strains comprised four lymphotropic wild-type isolates designated AB, DF, DL, and WTF. Since the downregulation of CD46 is caused by interaction with newly synthesized MV hemagglutinin (MV-H), we tested the capability of recombinant MV-H proteins to downregulate CD46. Recombinant MV-H proteins of MV strains Edmonston, Halle, and CM led to the down-regulation of CD46, whereas those of DL and WTF did not. This observed differential downregulation by different MV strains has profound consequences, since lack of CD46 on the cell surface leads to susceptibility of cells to complement lysis. These results suggest that lymphotropic wild-type strains of MV which do not downregulate CD46 may have an advantage for replication in vivo. The relatively weak immune response against attenuated vaccine strains of MV compared with wild-type strains might be related to this phenomenon.
最近，人们发现两种细胞表面分子，即CD46和肌动蛋白结合蛋白，在功能上与麻疹病毒（MV）对细胞的感染性相关。我们研究了MV野生型、亚急性硬化性全脑炎型及疫苗株的受体使用情况，以及它们感染后对CD46下调的影响。我们发现，用针对CD46的抗体可抑制所测试的全部19种MV株对人细胞系的感染。相比之下，并非所有MV株感染后都会导致CD46下调。CD46非下调株组包括四种嗜淋巴细胞野生型分离株，分别命名为AB、DF、DL和WTF。由于CD46的下调是由与新合成的MV血凝素（MV-H）相互作用引起的，我们测试了重组MV-H蛋白下调CD46的能力。Edmonston、Halle和CM株的重组MV-H蛋白可导致CD46下调，而DL和WTF株的则不能。不同MV株观察到的这种差异性下调具有深远影响，因为细胞表面缺乏CD46会导致细胞对补体裂解敏感。这些结果表明，不下调CD46的嗜淋巴细胞野生型MV株可能在体内复制方面具有优势。与野生型株相比，针对减毒MV疫苗株的免疫反应相对较弱可能与此现象有关。
