Mahmood T, Saridogan E, Smutna S, Habib A M, Djahanbakhch O
Academic Department of Obstetrics, Gynaecology and Reproductive Physiology, St Bartholomew's and the Royal London School of Medicine, The Royal London Hospital, Whitechapel, UK.
Hum Reprod. 1998 Nov;13(11):2991-4. doi: 10.1093/humrep/13.11.2991.
Using a method that detects variations in light intensity we have studied the effect of ovarian steroids on human Fallopian tube epithelial ciliary beat frequency in vitro. We have found that baseline ciliary beat frequency averages between 5-6 Hz. Cilia from ampullary segments of the Fallopian tube beat significantly faster (5.4 Hz+/-0.2) than those from fimbrial segments (4.8 Hz+/-0.2). There was no significant difference in baseline ciliary beat frequency at any other anatomical site in the Fallopian tube. Incubation with progesterone (10 micromol/l) suppresses human Fallopian tube epithelial ciliary beat frequency by 40-50%. This inhibition was observed at similar magnitudes in all Fallopian tubes studied irrespective of anatomical site. Progesterone-induced reductions in ciliary beat frequency were concentration dependent and prevented by the progesterone receptor antagonist mifepristone (RU486). Oestradiol alone (10 micromol/l) had no effect on ciliary beat frequency at any anatomical site in the Fallopian tube but did prevent the reduction in ciliary beat frequency seen with progesterone when tissues were incubated with these two steroids together.
我们使用一种检测光强度变化的方法，在体外研究了卵巢甾体激素对人输卵管上皮纤毛搏动频率的影响。我们发现基线纤毛搏动频率平均在5 - 6赫兹之间。输卵管壶腹部节段的纤毛搏动明显比伞部节段的更快（5.4赫兹±0.2）（伞部节段为4.8赫兹±0.2）。在输卵管的任何其他解剖部位，基线纤毛搏动频率均无显著差异。用孕酮（10微摩尔/升）孵育可使人类输卵管上皮纤毛搏动频率降低40 - 50%。在所有研究的输卵管中，无论解剖部位如何，均观察到类似程度的抑制作用。孕酮诱导的纤毛搏动频率降低呈浓度依赖性，且可被孕酮受体拮抗剂米非司酮（RU486）阻断。单独使用雌二醇（10微摩尔/升）对输卵管任何解剖部位的纤毛搏动频率均无影响，但当组织同时用这两种甾体激素孵育时，它确实能阻止孕酮引起的纤毛搏动频率降低。
