Department of Physiology, Osaka Medical College, Takatsuki, Japan.
Exp Physiol. 2010 Jul;95(7):819-28. doi: 10.1113/expphysiol.2010.052555. Epub 2010 Apr 1.
The ciliary beat frequency (CBF) of guinea-pig fimbria during the ovarian cycle was measured by video microscopy using a high-speed camera (500 Hz). In the follicular phase, with increasing concentrations of beta-oestradiol ([betaE(2)]) and a low concentration of progesterone ([PRG]), CBF increased from 13.5 to 16 Hz. In the ovulatory phase, with further increase of [betaE(2)], CBF decreased gradually from 16 to 13.5 Hz. In the early luteal phase, with low [PRG] and [betaE(2)], CBF increased to 17 Hz; however, in the middle luteal phase, with increasing [PRG], CBF decreased (12 Hz), and in the late luteal phase, with decreasing [PRG], CBF increased to 15 Hz. Then, in the resting phase, with low [betaE(2)] and [PRG], CBF decreased immediately to 14 Hz. The CBF of the fimbria was measured in guinea-pigs treated with beta-oestradiol benzoate (betaE(2)B) or medroxyprogesterone (mPRG). A low dose of betaE(2)B increased CBF to 14.5 Hz, whereas a high dose decreased it to 11 Hz. A betaE(2) receptor blocker, ICI-182,780, abolished the betaE(2)B-induced CBF changes and maintained CBF at 12.0 Hz. Medroxyprogesterone decreased CBF to 12.5 Hz, and mifepristone (a PRG receptor blocker) abolished the mPRG-induced CBF decrease and maintained CBF at 15 Hz. The addition of both blockers increased CBF to 18 Hz, suggesting that activation of betaE(2) or PRG receptors decreases the CBF of the fimbria. In conclusion, a moderate [betaE(2)] increase maintains a high CBF (15.5 Hz) in the follicular phase, and then further [betaE(2)] increase decreases CBF to 13.5 Hz in the ovulatory phase. In the early and late luteal phase, low [betaE(2)] and [PRG] increase CBF to 17 and 15 Hz, respectively, and in the middle luteal phase a high [PRG] decreases CBF (to 12 Hz). Thus, the CBF of the fimbria was controlled by signals via betaE(2) and PRG receptors in guinea-pigs.
豚鼠发情周期中子宫伞的纤毛拍频（CBF）通过高速摄像（500Hz）进行视频显微镜测量。在卵泡期，随着β-雌二醇（βE（2））浓度的增加和孕酮（PRG）浓度的降低，CBF 从 13.5Hz 增加到 16Hz。在排卵期，随着βE（2）的进一步增加，CBF 逐渐从 16Hz 降低到 13.5Hz。在黄体早期，随着 PRG 和βE（2）水平降低，CBF 增加到 17Hz；然而，在黄体中期，随着 PRG 水平增加，CBF 降低（12Hz），在黄体晚期，随着 PRG 水平降低，CBF 增加到 15Hz。然后，在静止期，随着βE（2）和 PRG 水平降低，CBF 立即降低到 14Hz。用苯甲酸雌二醇（βE（2）B）或甲羟孕酮（mPRG）处理豚鼠后测量子宫伞的 CBF。低剂量的βE（2）B 将 CBF 增加到 14.5Hz，而高剂量则将其降低到 11Hz。βE（2）受体阻滞剂 ICI-182780 消除了βE（2）B 诱导的 CBF 变化，并将 CBF 维持在 12.0Hz。甲羟孕酮将 CBF 降低到 12.5Hz，米非司酮（PRG 受体阻滞剂）消除了 mPRG 诱导的 CBF 降低，并将 CBF 维持在 15Hz。两种阻滞剂的加入将 CBF 增加到 18Hz，表明激活βE（2）或 PRG 受体降低了子宫伞的 CBF。总之，适度增加βE（2）水平在卵泡期维持高 CBF（15.5Hz），然后进一步增加βE（2）水平将排卵期的 CBF 降低至 13.5Hz。在黄体早期和晚期，低水平的βE（2）和 PRG 将 CBF 分别增加到 17Hz 和 15Hz，而在黄体中期，高水平的 PRG 将 CBF 降低（至 12Hz）。因此，豚鼠子宫伞的 CBF 受到βE（2）和 PRG 受体信号的控制。
