Meatherall R, Fraser A D
Biochemistry Laboratory, St. Boniface General Hospital, Winnipeg, Canada.
Ther Drug Monit. 1998 Dec;20(6):673-5. doi: 10.1097/00007691-199812000-00016.
Four healthy patient subjects were each given a single, 1-mg lorazepam tablet. Urine samples from all patient subjects were collected at 12 intervals (0-2, 2-5, 5-8, 8-11, 11-14, 14-24, 24-26, 26-29, 29-32, 32-35, 35-38, and 38-48 hours). An aliquot from each urine collection was screened using cloned enzyme donor immunoassay (CEDIA), enzyme-multiplied immunoassay technique (EMIT) II, EMIT dau, and fluorescence polarization immunoassay (FPIA) without and with hydrolysis using beta-glucuronidase. Using a 200 ng/mL calibrator cut-off, none of the four immunoassays gave a positive response before hydrolyzation of the urine samples. For offline hydrolysis using Helix pomatia beta-glucuronidase, 35, 3, 0, and 4 of 48 urine samples gave positive responses on the previously listed immunoassays. The CEDIA method also gave 32 of 48 positive responses for online hydrolysis using Escherichia coli beta-glucuronidase. Online hydrolysis can be conveniently automated by including the beta-glucuronidase in the first of the two reagents combined with the urine sample.
四名健康受试者每人服用一片1毫克的劳拉西泮片。在12个时间段（0 - 2、2 - 5、5 - 8、8 - 11、11 - 14、14 - 24、24 - 26、26 - 29、29 - 32、32 - 35、35 - 38和38 - 48小时）收集所有受试者的尿液样本。每次尿液收集的一份等分试样使用克隆酶供体免疫测定法（CEDIA）、酶倍增免疫测定技术（EMIT）II、EMIT dau和荧光偏振免疫测定法（FPIA）进行筛查，筛查时尿液样本不进行水解以及使用β - 葡萄糖醛酸酶进行水解。使用200 ng/mL的校准物截断值，在尿液样本水解前，这四种免疫测定法均未给出阳性反应。对于使用紫贻贝β - 葡萄糖醛酸酶进行离线水解，48份尿液样本中有35份、3份、0份和4份在上述免疫测定法中给出阳性反应。CEDIA方法对于使用大肠杆菌β - 葡萄糖醛酸酶进行在线水解的48份尿液样本中有32份给出阳性反应。通过将β - 葡萄糖醛酸酶包含在与尿液样本混合的两种试剂中的第一种中，在线水解可以方便地实现自动化。
