基于重复元件序列的聚合酶链反应对单核细胞增生李斯特菌菌株进行分型
Typing of Listeria monocytogenes strains by repetitive element sequence-based PCR.
作者信息
Jersek B, Gilot P, Gubina M, Klun N, Mehle J, Tcherneva E, Rijpens N, Herman L
机构信息
Centre of Agricultural Research, Department of Animal Product Quality (DVK), B-9090 Melle, Belgium.
出版信息
J Clin Microbiol. 1999 Jan;37(1):103-9. doi: 10.1128/JCM.37.1.103-109.1999.
Abstract
Listeria monocytogenes strains possess short repetitive extragenic palindromic (REP) elements and enterobacterial repetitive intergenic consensus (ERIC) sequences. We used repetitive element sequence-based PCR (rep-PCR) to evaluate the potential of REP and ERIC elements for typing L. monocytogenes strains isolated from humans, animals, and foods. On the basis of rep-PCR fingerprints, L. monocytogenes strains were divided into four major clusters matching origin of isolation. rep-PCR fingerprints of human and animal isolates were different from those of food isolates. Computer evaluation of rep-PCR fingerprints allowed discrimination among the tested serotypes 1/2a, 1/2b, 1/2c, 3b, and 4b within each major cluster. The index of discrimination calculated for 52 epidemiologically unrelated isolates of L. monocytogenes was 0.98 for REP- and ERIC-PCR. Our results suggest that rep-PCR can provide an alternative method for L. monocytogenes typing.
摘要
单核细胞增生李斯特菌菌株具有短的重复基因外回文序列（REP）和肠杆菌重复基因间共有序列（ERIC）。我们使用基于重复元件序列的聚合酶链反应（rep-PCR）来评估REP和ERIC元件用于对从人、动物和食品中分离出的单核细胞增生李斯特菌菌株进行分型的潜力。基于rep-PCR指纹图谱，单核细胞增生李斯特菌菌株被分为四个主要簇，与分离来源相匹配。人和动物分离株的rep-PCR指纹图谱与食品分离株的不同。对rep-PCR指纹图谱的计算机评估允许在每个主要簇内区分测试的血清型1/2a、1/2b、1/2c、3b和4b。对52株流行病学上不相关的单核细胞增生李斯特菌分离株计算的REP-PCR和ERIC-PCR的鉴别指数为0.98。我们的结果表明，rep-PCR可为单核细胞增生李斯特菌分型提供一种替代方法。
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