Domínguez M C, Herrera E
Biochem J. 1976 Aug 15;158(2):183-90. doi: 10.1042/bj1580183.
The uptake and utilization of [1-14C]glycerol was determined in pieces of rat epididymal fat-pads incubated in Krebs--Ringer bicarbonate buffer containing albumin. Insulin (200 muunits/ml), adrenaline (epinephrine; 0.5 mug/ml) and glucose (0, 5, 15 and 20 mM) were added to the medium. Changes in the specific radioactivity of the tracer during the incubation were taken into account in calculating the rate of glycerol utilization. Adrenaline decreased glycerol uptake, whereas insulin plus adrenaline increased it. The rate of incorporation of glycerol into glycerides was decreased by adrenaline and insulin, singly or together. Insulin increased the rate of formation of CO2 and fatty acids from glycerol. The formation of CO2 and fatty acids was further enhanced by insulin plus adrenaline. The decrease in glycerol uptake induced by adrenaline, the decrease in incorporation of glycerol into glycerides induced by insulin and insulin plus adrenaline and the synthesis of fatty acids were dependent on the presence of glucose in the medium. Thus insulin and adrenaline act on glycerol utilization in adipose tissue and some of their effects are mediated by action on glucose metabolism, but others are independent of this.
在含有白蛋白的 Krebs-Ringer 碳酸氢盐缓冲液中孵育的大鼠附睾脂肪垫切片中，测定了[1-14C]甘油的摄取和利用情况。向培养基中添加了胰岛素（200 微单位/毫升）、肾上腺素（0.5 微克/毫升）和葡萄糖（0、5、15 和 20 毫摩尔）。在计算甘油利用率时，考虑了孵育过程中示踪剂比放射性的变化。肾上腺素降低了甘油摄取，而胰岛素加肾上腺素则增加了甘油摄取。肾上腺素单独或与胰岛素一起降低了甘油掺入甘油酯的速率。胰岛素增加了甘油生成二氧化碳和脂肪酸的速率。胰岛素加肾上腺素进一步增强了二氧化碳和脂肪酸的生成。肾上腺素引起的甘油摄取减少、胰岛素和胰岛素加肾上腺素引起的甘油掺入甘油酯减少以及脂肪酸的合成均取决于培养基中葡萄糖的存在。因此，胰岛素和肾上腺素作用于脂肪组织中的甘油利用，它们的一些作用是通过对葡萄糖代谢的作用介导的，但其他作用则与此无关。
