Domínquez M C, Herrera E
Horm Metab Res. 1976 Jan;8(1):33-7. doi: 10.1055/s-0028-1093688.
The effects of 2-deoxy-D-glucose (2DG), oligomycin and theophylline on the in vitro production and metabolism of glycerol and its response to insulin and epinephrine were studied in epididymal fat pads from fed rats. 2-DG failed to affect basal or epinephrine stimulated glycerol production but it decreased the uptake of 1-14 C-glycerol by the tissue and its conversion to glyceride-glycerol. Oligomycin also failed to affect the basal production of glycerol but it inhibited the effect of epinephrine on this parameter as well as the uptake and utilization of 1-14-C-glycerol. Theophylline enhanced the production of glycerol by the tissue and this effect was not further augmented by epinephrine. Theophyline also inhibited the uptake and utilization of 1-14C-glycerol; the most pronounced effect of theophylline was observed in the formation of 14C-fatty acids from 1-14C-glycerol in the presence of glucose. Insulin, but not epinephrine, decreased the inhibitory effect of theophylline on glycerol utilization. It is concluded that these compounds affect more intensely the ability of adipose tissue to metabolize glycerol than to release it through lipolysis. The pathway for glycerol utilization in adipose tissue appears to be more sensitive to changes in the availability of ATP than the mechanisms responsible for the release of glycerol from the tissue.
研究了2-脱氧-D-葡萄糖（2DG）、寡霉素和茶碱对喂食大鼠附睾脂肪垫中甘油体外生成和代谢及其对胰岛素和肾上腺素反应的影响。2DG未能影响基础或肾上腺素刺激的甘油生成，但它降低了组织对1-14C-甘油的摄取及其向甘油酯甘油的转化。寡霉素也未能影响甘油的基础生成，但它抑制了肾上腺素对该参数的影响以及对1-14C-甘油的摄取和利用。茶碱增强了组织中甘油的生成，且肾上腺素并未进一步增强这种作用。茶碱还抑制了1-14C-甘油的摄取和利用；在有葡萄糖存在的情况下，观察到茶碱对由1-14C-甘油形成14C-脂肪酸的作用最为明显。胰岛素而非肾上腺素降低了茶碱对甘油利用的抑制作用。得出的结论是，这些化合物对脂肪组织代谢甘油能力的影响比对通过脂解作用释放甘油能力的影响更为强烈。脂肪组织中甘油利用途径似乎比负责从组织中释放甘油的机制对ATP可用性变化更为敏感。
