Jonjić N, Valković T, Lucin K, Iternicka Z, Krstulja M, Mustać E, Dobi-Babić R, Sasso F, Melato M
Medical Faculty, University of Rijeka, Croatia.
Anticancer Res. 1998 Sep-Oct;18(5B):3767-70.
Neovascularization, the growth and formation of capillary blood vessels, is an essential component of solid tumor growth and a critical step in metastasis. Tumor associated macrophages (TAMs) have several functions related to tumor biology including growth, proliferative rate, stroma formation and dissolution, and neovascularization. The aim of this study was to define the TAM and microvessel density (MD) in human invasive breast carcinoma NOS and to correlate their values with lymph node status, tumor size, tumor grade and mitotic activity index (MAI), and, finally, to determine whether MD is connected with TAMs. A total number of 57 invasive breast carcinomas NOS were processed for immunohistochemical analysis using mAb to F-VIII to visualize endothelial cells and mAb to CD68 antigens for macrophages. Statistical analysis showed only a positive correlation between TAMs and MAI (p = 0.004). These results support the notion that intensity of tumor angiogenesis does not provide additional prognostic significance, while TAMs may play a positive role in breast cancer micro system since they regulate tumor proliferation.
新生血管形成，即毛细血管的生长和形成，是实体瘤生长的重要组成部分，也是转移过程中的关键步骤。肿瘤相关巨噬细胞（TAM）具有多种与肿瘤生物学相关的功能，包括生长、增殖率、基质形成与溶解以及新生血管形成。本研究的目的是确定人浸润性乳腺癌NOS中的TAM和微血管密度（MD），并将它们的值与淋巴结状态、肿瘤大小、肿瘤分级和有丝分裂活性指数（MAI）相关联，最后确定MD是否与TAM相关。使用抗F-VIII单克隆抗体来可视化内皮细胞，使用抗CD68抗原单克隆抗体来检测巨噬细胞，对总共57例浸润性乳腺癌NOS进行免疫组织化学分析。统计分析显示TAM与MAI之间仅存在正相关（p = 0.004）。这些结果支持以下观点：肿瘤血管生成强度不具有额外的预后意义，而TAM可能在乳腺癌微系统中发挥积极作用，因为它们调节肿瘤增殖。
